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8 Cards in this Set
- Front
- Back
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What is the Polymerase Chain reaction?
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PCR
An invitro process Quickly amplifies DNA |
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For what is PCR used?
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Evolution research
pre-natal diagnosis crime scene investigation ESSENTIALLY TO AMPLIFY DNA |
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What are the steps of PCR
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Putting it all in the test tube
Heating Cooling DNA Polymerase steps in The Result |
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Outline Stage 1 of PCR
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The desired DNA with the gene of interest,
heat resistand DNA polymerase, free nucleotides AND a primer Are placed in an eppendorf tube |
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Outline Stage 2 of PCR
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The contents of the test tube are then heated to separate the complementary strands
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Outline Stage 3 of PFCR
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The contents of the tube are then cooled so that the primers can reform with the DNA via complementary base pairing
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Outline Stage 4 of PCR
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DNA Polymerase expands the primers by means of the free nucleotides; with the DNA acting as the parent strand
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Outline Stage 5 of PCR ie the result
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By the process of PCR the DNA has been doubled and the cycle repeats again
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