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5 Cards in this Set
- Front
- Back
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polymerase chain reaction
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making copies of a specific sequence of DNA
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primer
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short lengths of about 20 to 30 nucleotides, artifically synthesised, complements the nucleotide sequence which lies at the end of the target DNA
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the PCR reaction mixture contains:
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primer, DNA polymerase enzymes, deoxynucleotide phosphates, buffer
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buffer
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solution with the ability to absorb hydrogen ions, does not significantly change its pH when moderate amounts of acid or alkali are added
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polymerases
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enzymes that catalyse the formation of long-chain molecules (polymers)
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