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5 Cards in this Set

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polymerase chain reaction
making copies of a specific sequence of DNA
primer
short lengths of about 20 to 30 nucleotides, artifically synthesised, complements the nucleotide sequence which lies at the end of the target DNA
the PCR reaction mixture contains:
primer, DNA polymerase enzymes, deoxynucleotide phosphates, buffer
buffer
solution with the ability to absorb hydrogen ions, does not significantly change its pH when moderate amounts of acid or alkali are added
polymerases
enzymes that catalyse the formation of long-chain molecules (polymers)