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17 Cards in this Set

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What are the general transport guidelines?
1. Samples must be labeled
(Patient name, Medical record #/birth, Date, time, your initials)

2.Samples placed in leak-proof bag
(biohazard symbol, plus pouch for paper)

3. Samples remain upright
(reduce contamination from stopper, promote clot formation w/o additive present, reduce aerosol forming)

4. Sample transported to off-site by mail/courier must meet DOT regulations.
What are regulation made in the transport of samples to off-site location by mail/courier?
1. primary container wrapped in absorbent material and put in 2nd containter

2. 2nd container ---> shipping container

3. no extreme temp changes. (add dry ice)

4. Biohazard symbol on shipping container plus name of agent.
What is the corrective action for a plebotomist after collection?
The corrective action is to redraw
Factor that affect sample integrity.
Specimen integrity affected by:
- Method of transport

- Timing delays

- Temperature

- Movement/agitation

- Exposure to light

- Centrifugation methods
What is the time limit for routine samples?

Why?
Deliver sample within 45 minutes of being drawn.

CLSI recommends no more than 2 hours pass b/w collection and separation of cells from plasma/serum.
What is the time limit for STAT samples?
A “STAT” Request
- In cases of emergency

- Deliver immediately after draw

- Document the request appropriately
What are temperature requirements for most samples?
Most samples should be kept at room temperature
What are examples of samples that need to be warmed (37 C)?
Cold agglutinins and Cryofibrinogen
What are examples of samples that need to be chilled (in slurry)?
1.Blood gases

2. Lactic acid

3. Ionized calcium

4. Ammonia

5. Glucagon

6. Gastrin
What are examples of specimens that are affected by light?
Specimens that are light sensitive. They break down by light exposure.
- Bilirubin

- Vitamin B12

- Carotene
What are some common sample delivery methods?
Common delivery methods:
- Hand carry

- Hospital transportation department

- Pneumatic tube system

- Automated robot

- Commercial courier
What are the tasks of central processing?
The specimens are:
- identified

- Logged in

- sorted by department

- Centrifuged

- Aliquot preparation
What are centrifugation used for in central processing?
Centrifugation
- Centrifugal force separates cells from plasma/serum

- serum must clot completely prior to centrifugation

- Complete clotting takes 30-45 min.

- Faster with clot activator (15min)
What are the criteria for specimen rejection?
-Unlabeled specimens

- QNS specimens

- Hemolyzed specimens

- Incorrect anticoagulant for test

- Outdated tubes

- Improper handling (e.g. expose to UV light)

- Contaminated specimen (e.g. IV fluid)

- Serum not removed from cell within 2 hrs of collection.
What info are on requisition forms?
-- Patient identification (name, registration or identification number, location)

- Patient’s gender, date of birth or age

- Name of physician or legally authorized person ordering the test

- Tests requested

- Time and date of specimen collection

- Source of specimen

- Other pertinent clinical information when appropriate
What happens when requisitions is missing information?
Do not process the sample.
What is the corrective action log?
- Problems must be documented

- Corrective actions implemented and monitored

- Process review