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60 Cards in this Set
- Front
- Back
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what type of media is thioglycollate? |
enriched |
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what type of media is GN broth? |
selective for salmonella and shigella can inhibit NF |
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what type of media is CIN agar? |
selects for yersinia |
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what type of media is isovitalex? |
for fastidious organisms |
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what type of media is MAC? |
selective and differential selects for gram neg differentiates LF and non LF |
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what type of media is EMB? |
selective and differential (may be used instead of MAC) |
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what type of media is CHROMagar? |
selective and differential (organism specific) |
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what type of media is SSA agar? |
strep selective and enriched |
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what type of agar is BAP? |
enriched and differential (used to detect hemolytic rxns) |
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What type of agar is CHOC? |
enriched used to isolate fastidious organisms haemophilus and neisseria |
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What type of agar is CNA? |
enriched and selective selects for gram pos |
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what type of agar is PEA? |
enriched and selective selects for gram pos |
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what type of agar is MTM? |
enriched and selective selects for neisseria gonorrhoeae |
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what type of agar is XLD? |
selective and differential isolates salmonella and shigella can inhibit gram pos and NF |
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what type of media is Campy CVA? |
selective isolates campylobacter |
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what type of media is MSA? |
selective and differential selects for staph differentiates STA from STN |
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what type of media is Tinsdale agar? |
selective and differential isolates C. diphtheriae |
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what type of media is Cystine tellurite agar? |
tinsdale agar with 5% sheep blood |
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what type of media is loeffler media? |
non selective egg based used for stimulation of metachromatic granules |
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What type of media are CTA sugars? |
semisolid agar detects acid production of fermenters old test used to detect neisseria doesn't work because neisseria is oxidative |
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what type of agar is HE? |
selective and differential selects for salmonella and shigella can be used instead of XLD can inhibit normal flora |
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what type of media is SS agar? |
salmonella/shigella isolation highly selective and differential |
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what kind of media is SMAC agar? |
maconky agar with sorbitol selective and differential isolates sorbitol negative E. coli from stools isolates shiga toxin producing O157 strains |
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what kind of media is bismuth sulfite agar? |
highly selective and differential isolates salmonella can inhibit NF and shigella |
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what type of media is selective F broth? |
selective and enriched isolates salmonella and shigella inhibits NF |
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what type of media is KCN broth? |
selective and differential differentiates citrobacter freudii and salmonella |
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which medias isolate salmonella and shigella? |
XLD agar HE agar SS agar GN broth selective F broth |
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what type of media is bile esculin agar? |
selective and differential isolates group D strep and enterococcus |
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what are the components of bile esculin agar? |
bile salt: inhibits most gram positives esculin: differentiates group D strep from other strep groups |
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what are the possible results on bile esculin agar? |
blackening of the medium indicating hydrolysis of esculin: positive for group D strep or enterococcus |
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why is bile esculin agar selective and differential for group D strep and enterococcus? |
All group D strep and enterococci can grow in the presence of bile and also hydrolyze esculin |
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what are the components of bismuth sulfite agar? |
bismuth sulfite: inhibits NF and shigella brilliant green: inhibits NF and shigella ferrous sulfate: reacts to form a black precipitate |
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what are the possible results on bismuth sulfite agar? |
black/green colonies surrounded by brown/green sheen: salmonella growth |
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what are the components of EMB (eosin-methylene blue agar)? |
Eosin Y and methylene blue: inhibit gram positives lactose and sucrose: differentiate LF and non LF |
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what are the possible results on EMB? |
blue-black colonies with green metallic sheen (acid): rapid lactose fermenter blue/purple colonies: slow lactose fermenters colorless (alkaline): non fermenters |
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what are the components of GN broth? |
desoxycholate and citrate salts: inhibit growth of gram positives increased mannitol: assists growth of salmonella and shigella for 4 hours |
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what are the components of HE (HekToen enteric) agar? |
bile salts inhibit gram positives and some gram negative NF PH indicators/inhibitors: bromthymol blue and acid fuchsin lactose, sucrose, and salicin: differentiate LF and non LF ferric salts: detect H2S gas |
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what are the possible results on HE agar: |
orange/salmon(acid): LF clear(alkaline): non LF black: H2S producer |
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what are the components of MAC agar? |
bile salts and crystal violet: inhibit gram positives lactose: differentiates LF and non LF PH indicator: neutral red |
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what are the possible results on MAC (macConkey)agar? |
pink colonies (acid): LF clear colonies(alkaline): non LF clear at 24 hrs then pink at 48hrs: late LF |
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what are the components of MSA (mannitol salt agar)? |
7.5% NaCl: inhibits most gram pos and gram neg mannitol: differentiates STA from STN PH indicator: phenol red |
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what are the possible results on MSA? |
red colonies (alkaline): non mannitol fermenter yellow colonies(acid): mannitol fermenter |
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what are the components of XLD? |
sodium desoxycholate: inhibits gram pos and gram neg cocci
lactose, sucrose, xylose: differentiates salmonella and shigella lysine: differentiates lysine decarboxylating organisms pH indicator: phenol red sodium thiosulfate Ferric ammonium sulfate: H2S indicator |
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what are the possible results on XLD? |
yellow colonies(acid): LF red colonies (alkaline): non LF or lysine decarboxylase producers black: H2S producers |
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what are the components of SS agar? |
bile salts, sodium citrate, brilliant green: inhibit gram pos lactose: differentiates LF and non LF pH indicator: phenol red sodium thiosulfate: sulfur source ferric ammonium sulfate: H2S indicator |
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what are the possible results on SS agar? |
pink colonies (acid): lactose fermenter black colonies: H2S producer clear colonies (alkaline): non LF |
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what are the components of CIN (cefsoludin irgasin novobiocin) agar? |
crystal violet, bile salt, antibiotics: inhibit all other bacteria mannitol: differentiates yersinia pH indicator: phenol red |
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what are the possible results on CIN agar? |
pink colonies (acid): mannitol fermenter (yersinia) clear colonies (alkaline): non mannitol fermenter |
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what are the components of SMAC? (MAC with sorbitol) |
crystal violet and bile salts: inhibit enterococci and other gram positives sorbitol differentiates pathogenic E. coli from other normal gut flora pH indicator: neutral red |
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what are the possible results on SMAC? |
pink colonies: sorbitol positive (E coli) clear colonies: sorbitol negative |
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what are the components of CHROMagar for E. coli? |
proprietary chromogenic mixtures and antibiotics: select for E. coli O157:H7 |
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what are the possible results on CHROMagar for E. coli? |
mauve colonies: E coli O157:H7 blue colonies: other E coli clear colonies: other enterics |
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what are the components of selenite F broth? |
selenite: inhibits gram positives and neg NF lactose and phosphate buffers: maintain pH and reduce toxicity of alkaline products of selenite reduction cysteine: enhances recovery of salmonella |
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what are the components of KCN broth? |
cyanide ion: inhibits bacterial respiration salts: inhibit gram positives |
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what type of Agar is Tinsdale's Agar? |
selective and differential isolates C. diphtheria |
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what are the components of tinsdale's agar? |
potassium tellurite: inhibits NF |
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what are the possible results on tinsdale's agar? |
black-brown colonies w/ a brown halo: possible C. dyphtheriae |
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what are the components of cysteine-tellurite agar? |
-tinsdale agar with 5% sheep blood
-cysteine and tellurite inhibit gram neg and NF |
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what are the possible results on cysteine-tellurite agar? |
black-grey colonies: C. dyphtheriae |
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what type of media is Loeffler's media |
non-selective egg based detection of metachromatic granules |
