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41 Cards in this Set

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What are 5 possible reasons for layering success?

1. Maintaining a Physical attachment of stem to the mother plant. Xylem remains intact.


2. Accumulation of photosynthesis & hormones in the rooting area. Girdling & exogenous auxins may be needed.


3. Light exclusion in the root zone. Blanching and etoliation.


4. Invigoration & Rejuvenation. Cutting back stock plants results in rapid regrowth(invigoration)


Reversal of mature growth to juvenile form enhances rooting.


5. Seasonal Patterns. Often started in early spring with roots developing as growth resumes.

What is layering?

A way of rooting cuttings on a stem while still attached to the plant.

What common technique has replaced layering in the nursery trade?

Cuttings

What reason allows for a continuous supply of water and minerals to pass through the intact xylem to the rooting area?

Guttation

In most cases, in which season is layering typically done?

Spring

What is the name if the process in which an entire ring of bark is removed from the stem during air layering?

Girdling

What is another name for Mound layering?

Stooling

What is the name if the shoots that are produced during Mound layering?

Stool shoots/ layer

Which species is most often associated with Tip layering?

Black berry

Which plant typically produces runners?

Strawberries

Which fruit tree is typically propagated from offshoots?

Date Palm

What is the name of the technique in which herbaceous perennials are split into separate parts with stem & root attached?

Crown Division

As a general rule when should fall flowering perennials be divided?

Spring

When should potted plants be divided?

When root bound

List 6 plant modifications resulting in Natural layering.

Tip layering, runners, stolons, offsets, suckers, crown division

Describe micro propagation.

Micro propagation is a form of tissue culture to propagate new plants in an aseptic ultra controlled environment within the culture vessel.

Micro propagation is commonly referred to by what term?

Tissue culture

According to the textbook, what are 5 advantages of micro propagation?

1. Mass propagation of specific clones


2. Production of pathogen free plants


3. Clonal propagation of parental stock(inbred) for hybrid seed


4. Year-round nursery production


5. Germplasm preservation

6 disadvantages of micro propagation.

1. Require expensive facilities


2. High labor cost


3. High volume distribution required


4. Infestation can cause quick/ high losses


5. Variability of off types


6. Economics/ marketing

Why would micro propagation be used on a new cultivar that has a high market demand?

Mass production

Where should the tissue culture facility be located in the nursery?

Away from main nursery

What are the 3 areas in a tissue culture facility?

1. Preparation area


2. Transfer area


3. Growing area

Name 7 items usually found in the preparation area.

Scales, stirrers, media dispenser, autoclave, heating plates, pH meters, filters, water purification

What is the number one requirement for the transfer area?

Must be STERILE

List 5 containers used for growing cultures.

Test tubes, glass jars, petri dishes, plastic containers, flasks

What are the 3 categories of ingredients found in culture media?

Gelling, organic, inorganic

What is n example of an inorganic salt?


Nitrogen, potassium

What category would sucrose & vitamins fall under?

Organic

Name 2 gelling agents.

Agar, gelrite, liquid solution

Which is most commonly used as a gelling agent?

Agar

What is the main purpose of Agar in a culture media?

Anchorage, Nutrients

What are the 4 stages of micro propagation?

1. Establishment


2. Shoot Multiplication


3. Root Formation


4. Acclimatization

What stage would you subculture the developing explants?

2- Shoot Multiplication

In which stage do you disinfest the explants?

1- Establishment

What is a plantlet?

A small or young plant

What is the most common disinfesting solution?

Bleach, alcohol

Is the same media used for all stages?

No

What is the purpose of stage 3?

Root Formation

Do more shoots or roots develop during the multiplication stage?

Shoots

What process must be done to plantlets once they're well rooted in order to survive?

Acclimatization

The method of rooting micro cuttings where they're rooted into plug cells containing a greenhouse medium is called?

Ex-vitro