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27 Cards in this Set

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Transformation

A piece of donor DNA is taken up by a recipient bacterium and through recombination it is incorporated into the recipient's DNA. This is a source of genetic variation.

Three virus genes

Coat proteins: protection of nucleic acid.


Replicase: codes for nucleic acid replication, needed for continuation of genetic material.


Movement protein: moving around host organism.

Conjugation

Bacterial mating where genetic info between two bacteria is passed. Source of genetic variation.

Crown gall

Agrobacterium tumefaciens

Pathovar

In bacteria a subspecies or group of strains that can infect only plants within a certain genus or species.

Viruliferous

Said of a vector containing a virus and capable of transmitting it.

Viroids

Small, low molecular weight RNA that can infect plant cells, replicate themselves and cause disease.

Virus

A submicroscopic obligate parasite consisting of nucleic acid and protein.

Encapsidation

Coat protein gene responsible for this

4 Virus symptom development

Systemic, local lesions, latent virus live symptomless on a host called symptomless carrier, masked.

Virus symptoms

Mosaics and ring spots. Also stunting, yellows, pitting, distortion

Insect vectors

Nonpersistent viruses

Stylet-borne viruses persist in the vector for only a few to several hours.

Semipersistent viruses

Viruses persist in vector for 1-4 days

Circulative and persistent viruses

Viruses that are acquired by their vectors through their mouth parts accumulate internally and then are passed through tissues of the vector and introduced into plants again via the mouth parts of the vector

Virus shapes

Bacterial morphology

Cell wall, slime layer or capsule maybe, flagella (either polar or peritrichous, all over), most rod shaped except for filamentous Strptomyces. Large circular chromosome, occasionally small circular plasmid. Reproduce by binary fission, also do conjugation.

Identifying bacteria

Gram staining, nutritionally different media, enzymes produced, pathogenicity, serological methods, pcr

Blight

A disease characterized by general and rapid killing of leaves, flowers and stems.

Burrill

1878, first to transmit disease in plants in fire blight. Father of phytobacteriology

Mary dell Chilton

1977 agrobacterium tumefaciens physically transfers part of its plasmid

Bacterial growth

Genetic variation in bacteria

Transformation (-physical uptake of a piece of DNA or whole plasmid DNA.


Conjugation


Transduction-phage-mediated DNA transfer

Bacteria

Mostly facultative saprophytes

Ice nucleation

P. Syringae, Steve lindow bacteria deleted gene

Beijerinck

Father of virology, contagium vivum fluidum

Icosahedron

20 sided virus