Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
11 Cards in this Set
- Front
- Back
|
Prussian blue
|
-detects ferric(Fe3+) iron
-tissue is treated with acidic potassium ferrocyanide and ferric ions in the tissue form a blue pigment called prussian blue (ferric ferrocyanide). -Nuclear fast red is counterstain -hemosiderin=iron |
|
|
Turnbull blue
|
-detects ferrous(Fe2+) ions, which are toxic
-tissue is treated with acidic potassium ferricyanide and the ferrous ions in the tissue form a blue pigment called Turnbull blue (ferrous ferricyanide) -Nuclear fast red is counterstain |
|
|
Schmorl
|
-detects reducing substances such as melanin & argentaffin granules
-reducing agents reduce the ferric ions in the staining solution to ferrous ions, which combines with the ferricyanide in the staining solution creating Turnbull blue (ferrous ferricyanide) -counterstain is mayer mucicarmine -reducing substances=blue; goblet cells=rose; background=yellow-green |
|
|
Fontana-Masson
|
-detects melanin & argentaffin granules
-silver nitrate impregnation -melanin & argentaffin granules=black; nuclei=pink |
|
|
Grimelius
|
-demonstrates argyrophil granules in neurosecretory tumors
-silver nitrate impregnation -argentaffin & argyrophil=dark brown to black; nuclei=red; backgournd=pale yellow-brown |
|
|
Churukian-Schenk
|
-demonstrates argyrophil granules in neurosecretory tumors
-silver nitrate impregnation -argentaffin & argyrophil=black; nuclei=red; backgournd=pale yellow-brown |
|
|
Gomori methenamine silver
|
-for urates
-urates stained black |
|
|
Hall (Fouchet)
|
-demonstrates bilirubin (bile) in tissue
-Fouchet reagent= trichloracetic acid-ferric chloride solution -van Gieson used as counterstain -bile=green; background=yellow |
|
|
von Kossa
|
-detects calcium
-silver nitrate stain that uses UV light as a reducer -indirect b/c silver reacts w/the anions (carbonate & phosphate) of the calcium salts -Nuclear fast red is counterstain -calcium salts=black; background=red |
|
|
Alizarin red S
|
-detects calcium
-calcium deposits=orange-red -reaction product is birefringent |
|
|
Rhodanine
|
-detects copper, esp. in liver, in Wilson's disease
-copper=bright red to red yellow; nuclei=light blue |
