Physiology 534 Exam Ii: Bone & Cartilage Lab

Front 1

Is cartilage vascular or avascular?

Back 1

Avascular

Which tissue is avascular?

Front 2

What are the specialized cells of cartilage called?

Back 2

Chondrocytes

Which tissue are these specialized for?

Front 3

Why do chondrocytes have pale or vacuolated areas?

Back 3

Cytoplasm has glycogen, lipid, and large golgi apparatus

This space with the above components are found in what cell?

Front 4

Why do chondrocytes have a lacuna?

Back 4

During preparation, glycogen and lipids are lost and the cell will shrink leaving a surrounding space.

Front 5

Is the lacuna in chondrocytes seen in LM or EM?

Back 5

LM only!

Front 6

The 3 Cartilages all have what type of collagen fibers?

Back 6

Type II

Front 7

What color is the ECM of cartilage in H&E staining?

Back 7

Basophilic because the ECM contains sulfate groups on its proteoglycans which bind hemotoxylin.

Front 8

Why is the lacuna typically more intense basophilic?

Back 8

Because sulfate groups are present in highest concentrations around the lacunae.

Front 9

Can you tell the difference by looking at staining an old person vs. a young person?

Back 9

Yes, staining of cartilage from older individuals are less intensely basophilic than young individuals.

Front 10

What are the 3 types of cartilage?

Back 10

Hyaline, Elastic, Fibrocartilage

Front 11

What tissue is found in the Fetal skeletal tissue, epiphyseal plates, articular surface of synovial joints, costal cartilages of the rib cag, cartilages of the ansal cavity, laryn, rings of trachea and plates in bronchi

Back 11

Hyaline Cartilage

Front 12

What tissue is found in the Pinna of external ear, external acoustic meatus, auditory tube, cartilages of larynx

Back 12

Elastic Cartilage

Front 13

What tissue is found in the IV disks, pubic symphysis, knee joint

Back 13

Fibrocartilage

Front 14

Which cartilage provides resistance to compression, cushioning, structural support?

Back 14

Hyaline

Front 15

Which cartilage provides flexible support?

Back 15

Elastic

Front 16

Which cartilage resists deformation under stress?

Back 16

Fibrocartilage

Front 17

Which cartilages have pericondrium?

Back 17

Hyaline and Elastic

Front 18

Which cartilages undergo calcification?

Back 18

Hyaline and Fibrocartilage

Front 19

What are the major cell types of the cartilages?

Back 19

Chondroblasts and chondrocytes

Front 20

Which cartilage contains fibroblasts?

Back 20

Fibrocartilage

Front 21

Which cartilage contains Type II collagen fibrils

Back 21

All: hyaline, elastic, and fibrocartilage

Front 22

Which cartilage contains Type II and elastic fibers?

Back 22

Elastic cartilage

Front 23

Which cartilage contains Type II and Type I collage fibers

Back 23

Fibrocartilage

Front 24

How do the 3 cartilages grow?

Back 24

Interstitially and appositionally, very limited in adults

Front 25

Is the repair capability good for the 3 cartilages?

Back 25

Very limited capability, commonly forms scar resulting in fibrocartilage formation.

Front 26

Which cartilage has perichondrium containing blood vessels surrounding non-articular cartilage?

Back 26

Hyaline

Front 27

Which cartilage has chondrocytes in isogenic pairs?

Back 27

Hyaline

Front 28

In which cartilage are the chondrocytes closer together (less matrix) than in hyaline?

Back 28

Elastic

Front 29

Which cartilage has ECM that includes fine elastic fibers and lamellae?

Back 29

Elastic

Front 30

In which cartilage can deposits of adipose tissue occur?

Back 30

Elastic

Front 31

Which cartilage has chondrocytes that are sparse, arranged linearly, and separated by thick collagen bundles?

Back 31

Fibrocartilage

Front 32

Which cartilage has ECM that resembles Dense CT?

Back 32

Fibrocartilage

Front 33

Ground bone is used to prepare?

Back 33

Matrix organization-osteons, cells are destroyed, can use dyes such as India ink

Front 34

Decalcified bone is used to prepare?

Back 34

Cells but treated with acids to dissolve inorganic components, lamella preserved, can use to see blood vessels, and periosteum

Front 35

Which cell of the bone secrets collagen and ground substance of osteoid?

Back 35

Osteoblasts

Front 36

Which cells are cuboidal or polygonal and located on the surface of the forming bone?

Back 36

Osteoblasts

Front 37

Which cell differentiates from osteoblasts, surrounded by matrix, shrunk, and surrounded by a TRUE lacuna?

Back 37

Osteocytes

Front 38

Which cell removes bone during development?

Back 38

Osteoclasts

Front 39

How is the cytoplasm stained in Osteoblasts?

Back 39

Basophilic

Front 40

How is the cytoplasm stained in Osteoclasts?

Back 40

Eosinophilic

Front 41

Which cell has multiple nuclei and present and contains Howship's lacuna?

Back 41

Osteoclasts

Front 42

How is the bone matrix stained?

Back 42

Eosinophilic

Front 43

Which has Golgi and RER (Obsteoblasts or Osteocytes?)

Back 43

Osteoblasts

Front 44

Matrix of developing bone contains what staining?

Back 44

Both basophilic and eosinophilic

Front 45

What are the zones of Endochondral bone formation?

Back 45

Zone of reserve cartilage
Zone of proliferation
Zone of maturation/hypertrophy
Zone of Calcified cartilage
Zone of resorption
Zone of ossification

Front 46

What are intramembranous bone formation?

Back 46

Limited to those bones that are not required to perform an early supporting function

Front 47

What zone has chondrocytes with relatively little intervening matrix?

Back 47

Zone of reserve cartilage

Front 48

What zone contains parallel stacks of chondrocytes, daughter cells resulting from rapid mitosis

Back 48

Zone of proliferation

Front 49

What zone contains enlarged chondrocytes with pale cytoplasm (glycogen)

Back 49

Zone of maturation/hypertrophy

Front 50

What zone has chondrocytes die, lacunae are empty, and matrix calcifies (basophilic)

Back 50

Zone of calcified cartilage

Front 51

What zone has blood vessels and connective tissue invade area of dying chondrocytes?

Back 51

Zone of resorption

Front 52

What zone has bone cells that are present and ossified matrix is eosinophilic

Back 52

Zone of ossification