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55 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Includes lenses to enlarge objects of study |
Magnifying parts |
Aka OBJECTIVES |
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Has variable magnification |
Eyepiece |
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5x magnification |
Scanner |
Used to study details of LARGE ORGANISM |
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10x magnification |
LPO |
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40x magnification |
HPO |
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100x magnification |
Oil Immersion Objective |
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Modifies the light that illuminates object |
Illuminating Parts |
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Concentrates light |
Abbe condenser |
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Reflects light |
Mirror |
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Regulates the amount of light |
Iris diaphragm |
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Inversely proportional to magnification |
Wavelength of light |
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Supports the magnifying & illuminating Parts |
Mechanical parts |
Used to focus lenses |
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Supports ocular object |
Draw tube |
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Vacuum of air to prevent distortion of image |
Body tube |
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Used to change objectives |
Revolving nosepiece |
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Protects objectives from dusts |
Dust shield |
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Support the slide |
Stage w/ stage clips |
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To focus under low magnification |
Coarse adjustment knob |
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To focus under high magnification |
Fine adjustment knob |
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Supports microscope |
Base |
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Ease in examining specimen |
Inclination joint |
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Facilitates changing of objectives |
Forette |
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DIRECT IMAGING TECHNIQUES |
Light Field Microscopy Dark Field Microscopy |
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INDIRECT IMAGING TECHNIQUE |
UVL Microscopy Flourescent Microscopy Phase Contrast Microscopy Electron Microscopy |
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Used to study living/prepared specimens |
Light Field Microscopy |
1000-2000x |
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Important for study of spicochaetes |
Dark Field Microscopy |
1000-2000x |
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Use of UVL with a shorter WL |
UVL microscopy |
2000-4000x |
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Makes use of flourescent dye auramine. Reacts with UVL and produces BRIGHT YELLOW coloration. |
Flourescent Microscopy |
2000-4000x |
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Makes use of metallic stains like PHSOvPHOTUNGSTEN DYE. |
Electron Microscopy |
100 000- 200 000x |
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Organism must undergo lyophillization also known as free dry technique |
Electron Microscopy |
Dehydration of the specimen Aka, only microscope that can visualize viruses |
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Staining technique |
SWAB SMEAR FIX |
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Staining technique that uses a single dye, usually basic dye |
Simple Staining Technique |
Gives bacterial morphology |
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Round/spherical bacteria |
COCCI |
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Rod shaped/cigar shaped bacteria |
BACILLI |
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Coiled bacteria |
Spirals |
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Coiled |
Spirilla |
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Flexible coiled |
Spirillum |
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W/ axial filament |
Spirochaetes |
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Bacteria in chains |
Strepto |
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Bacteria in clusters |
Staphylo |
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Bacteria in pairs |
Diplo |
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Bacteria by 4s |
Tetra |
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Makes use of 2 or more dyes to differentiate one type of organidm fr another |
Differential Staining Technique |
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Used to classify microorg |
Gram Staining |
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Primary strain of Gram Staining |
Crystal Violet (BLUE) |
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Forms a complex with primary stain to w/c some org have affinity |
Gram's iodine |
Mordant of Gram Staining |
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Decolorizing agent |
Acidic alcohol |
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Counterstain (RED) |
Safranin |
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All COCCI are gram + except _____ |
NEISSERIA |
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All BACILLI are gram - except ______ |
Myobacterium, cornybacterium, bacillus & clostridium |
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Used to identify myobacterium (TB) |
Ziehl Nielsen Method |
Aka ACID FAST STAINING |
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Primary stain of Acid Fast Staining |
Carbolfuschine RED |
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Mordant of acid fast staining |
Heat |
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Decolorizing agent of acid fast staining |
Acid alcohol |
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Counterstain of acid fast staining |
Methylene blue |
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