Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
10 Cards in this Set
- Front
- Back
|
Acid-Fast Stain
|
Mycobacteria
Reagent: 1 (basic fuchsin/phenol/water), Acid/Alcohol, Counter (Meth. blue/water). |
|
|
Capsule Stain
|
S. pneumoniae, H. influenzae, K. pneumonaie
Reagent: India ink |
|
|
Flourochrome Acid-Fast Stain
|
Mycobacteria
Reagents: Auramine-Rhodamine T in phenol/glycerol/potassium permanganate |
|
|
Metachromatic Granule Stain
|
C. diptheriae
Reagent: Meth. blue/ethanol/water |
|
|
Spore Stain
|
Clostridium/Bacillus
Reagents: 1 (malachite green/water) counter (safranin O/ethanol/ water) |
|
|
Acid-fast stain
|
Dye is forced into cell using heat, remains inside during decolorizing. Acid bacilli will be pink, others blue.
|
|
|
Capsule Stain
|
Ink forms dark background and capsule stands out as transparent zone.
|
|
|
Fluorochrome Acid-Fast Stain
|
Decolorized for 2-3 minutes~~counterstained w/potassium permanganate 4-5 minutes.
|
|
|
Metachromatic granule stain
|
Positively charged methylene ion interacts w/negatively charged phosphate groups. Uses Loeffler's methylene blue, positive cells have blue granules
|
|
|
Spore Stain
|
Uses 72 hour culture.
Green in pink cytoplasmic background. |
