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15 Cards in this Set
- Front
- Back
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Acid v. Sugar Differentiation
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specializing Staphylococcus (Mannitol) and Neisseria & enterics
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S. Aureus
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Ferments glucose anaerobically
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N. gonorrhoeae
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Ferments only glucose
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N. Meningitis
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ferments glucose and maltose
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Phenol Red broth
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Media used for acid v. sugar differentiation tests
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Differentiates Staphylococcus from Streptococcus
Release of oxygen from hydrogen peroxide |
Catalase Differentiation
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fermentation of glucose, lactose, or sucrose
production of gas and hydrogen sulfide |
triple sugar ion reaction
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Glucose only fermenters + TSI
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cause pH to change through out the slant, and acids at top are oxidized rapidly & phenol red indicator remains neutral or alkaline
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H2S and TSI
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Ferrous ammonium sulfate and sodium thiosulfate in medium react w/H2S to form black ferrous sulfide
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Glucose fermentation to acid
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methyle red tests
red if pH below 4.5 differentiates E. coli from Enterbacter aerogenes |
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factor v
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nicotinamide adenine dinucleotide (NAD)
required for growth of Haemophili (BA) |
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pathogenic enterics
salmonella, shigella, proteus lack b-galactosidase & don't ferment lactose MacConkey |
Lactose-negative differentiation
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TSI Rxn:
yellow/acid butt yellow/acid slant |
glucose + lactose feremented
(E. coli) |
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TSI Rxn:
yellow/acid butt orange/pink/neutral/basic slant |
glucose only fermented (Salmonella, Shigella)
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TSI Rxn:
orange/red/neutral butt orange/red/neutral slant |
no fermentation
(pseudomonas) |
