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15 Cards in this Set

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Acid v. Sugar Differentiation
specializing Staphylococcus (Mannitol) and Neisseria & enterics
S. Aureus
Ferments glucose anaerobically
N. gonorrhoeae
Ferments only glucose
N. Meningitis
ferments glucose and maltose
Phenol Red broth
Media used for acid v. sugar differentiation tests
Differentiates Staphylococcus from Streptococcus
Release of oxygen from hydrogen peroxide
Catalase Differentiation
fermentation of glucose, lactose, or sucrose
production of gas and hydrogen sulfide
triple sugar ion reaction
Glucose only fermenters + TSI
cause pH to change through out the slant, and acids at top are oxidized rapidly & phenol red indicator remains neutral or alkaline
H2S and TSI
Ferrous ammonium sulfate and sodium thiosulfate in medium react w/H2S to form black ferrous sulfide
Glucose fermentation to acid
methyle red tests
red if pH below 4.5
differentiates E. coli from Enterbacter aerogenes
factor v
nicotinamide adenine dinucleotide (NAD)
required for growth of Haemophili (BA)
pathogenic enterics
salmonella, shigella, proteus lack b-galactosidase & don't ferment lactose
MacConkey
Lactose-negative differentiation
TSI Rxn:
yellow/acid butt
yellow/acid slant
glucose + lactose feremented
(E. coli)
TSI Rxn:
yellow/acid butt
orange/pink/neutral/basic slant
glucose only fermented (Salmonella, Shigella)
TSI Rxn:
orange/red/neutral butt
orange/red/neutral slant
no fermentation
(pseudomonas)