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17 Cards in this Set
- Front
- Back
General features of ALL Staphylococcus species
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Gram +
Catalase + non-motile non-spore forming FACULTATIVE anaerobe tolerates high NaCl conc. |
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Virulence factors of S. aureus
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Coagulase
Leukocidin Entertoxins Lipases Hyaluronidase Protein A Hemolysins |
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6 manifestations of disease caused by S. aureus
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Skin infections
Food Poisoning Toxic Shock Syndrome Pneumonia Osteomyelits Gastroenteritis |
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General characteristics of S. epidermidis
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normal flora of skin and external mucosa, low virulence
Catalase + like all other Staph Coagulase - Novobiocin susceptible |
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3 diseases caused by S. epidermidis
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endocarditis
infections of prosthetics infections of IV site - sepsis |
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General characteristics of S. saprophyticus
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Assoc. w/ UTIs in young, sexually active females. unique binding property to epithelium of urethra or bladder
Catalase + like all other staph Coagulase - Novobiocin RESISTANT |
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IDENTIFICATION OF STAPHYLOCOCCUS:
GRAM STAIN |
ALL CLINICALLY RELEVANT STAPH SPECIES ARE GRAM ++POSITIVE++
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IDENTIFICATION OF STAPHYLOCOCCUS:
AGAR GROWTH (SBA, Mannitol Salt) |
SBA - White colonies
Mannitol Salt 7.5% NaCl - ALL Staph can grow - Yellow colonies |
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IDENTIFICATION OF STAPHYLOCOCCUS:
Mannitol fermentation (S. aureus, epidermidis, saprophyticus) |
MANNITOL FERMENTATION:
S. aureus + S. epidermidis - S. saprophyticus +/- |
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IDENTIFICATION OF STAPHYLOCOCCUS:
Catalase |
ALL CLINICALLY RELEVANT STAPH SPECIES ARE CATALASE ++POSITIVE++
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IDENTIFICATION OF STAPHYLOCOCCUS:
Coagulase |
S. aureus +
S. epidermidis - S. saprophyticus - |
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IDENTIFICATION OF STAPHYLOCOCCUS:
Novobiocin resistance |
S. epidermidis S
S. saprophyticus R |
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Staph that are Tube coagulase positive:
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S. aureus
S. intermedius S. hyicus S. delphini |
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Staph that are Slide or clumping factor positive
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S. aureus
S. intermedius S. lugdunensis S. schleiferi |
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Coagulase negative GPC:
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S. epidermidis
S. saprophyticus other Staph species Micrococcus sp. Stomatococcus (Rothia) |
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Staphylococcus intermedius
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-normal flora of dogs: DOG BITE INFECTIONS
-coag neg @ 24hr; VP neg (S. aureus +) |
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Rothia mucilaginosus
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Opportunistic infections
endocarditis in IVDA's -encapsulated, mucoid colonies -CATALASE NEG OR WK POS -Glucose fermenting (Micrococcus -) -no growth 5% NaCl (staph and micro are +) |