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17 Cards in this Set

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General features of ALL Staphylococcus species
Gram +
Catalase +
non-motile
non-spore forming
FACULTATIVE anaerobe
tolerates high NaCl conc.
Virulence factors of S. aureus
Coagulase
Leukocidin
Entertoxins
Lipases
Hyaluronidase
Protein A
Hemolysins
6 manifestations of disease caused by S. aureus
Skin infections
Food Poisoning
Toxic Shock Syndrome
Pneumonia
Osteomyelits
Gastroenteritis
General characteristics of S. epidermidis
normal flora of skin and external mucosa, low virulence
Catalase + like all other Staph
Coagulase -
Novobiocin susceptible
3 diseases caused by S. epidermidis
endocarditis
infections of prosthetics
infections of IV site - sepsis
General characteristics of S. saprophyticus
Assoc. w/ UTIs in young, sexually active females. unique binding property to epithelium of urethra or bladder
Catalase + like all other staph
Coagulase -
Novobiocin RESISTANT
IDENTIFICATION OF STAPHYLOCOCCUS:

GRAM STAIN
ALL CLINICALLY RELEVANT STAPH SPECIES ARE GRAM ++POSITIVE++
IDENTIFICATION OF STAPHYLOCOCCUS:

AGAR GROWTH (SBA, Mannitol Salt)
SBA - White colonies

Mannitol Salt 7.5% NaCl - ALL Staph can grow - Yellow colonies
IDENTIFICATION OF STAPHYLOCOCCUS:

Mannitol fermentation
(S. aureus, epidermidis, saprophyticus)
MANNITOL FERMENTATION:

S. aureus +
S. epidermidis -
S. saprophyticus +/-
IDENTIFICATION OF STAPHYLOCOCCUS:

Catalase
ALL CLINICALLY RELEVANT STAPH SPECIES ARE CATALASE ++POSITIVE++
IDENTIFICATION OF STAPHYLOCOCCUS:

Coagulase
S. aureus +
S. epidermidis -
S. saprophyticus -
IDENTIFICATION OF STAPHYLOCOCCUS:

Novobiocin resistance
S. epidermidis S
S. saprophyticus R
Staph that are Tube coagulase positive:
S. aureus
S. intermedius
S. hyicus
S. delphini
Staph that are Slide or clumping factor positive
S. aureus
S. intermedius
S. lugdunensis
S. schleiferi
Coagulase negative GPC:
S. epidermidis
S. saprophyticus
other Staph species
Micrococcus sp.
Stomatococcus (Rothia)
Staphylococcus intermedius
-normal flora of dogs: DOG BITE INFECTIONS
-coag neg @ 24hr; VP neg (S. aureus +)
Rothia mucilaginosus
Opportunistic infections
endocarditis in IVDA's
-encapsulated, mucoid colonies
-CATALASE NEG OR WK POS
-Glucose fermenting (Micrococcus -)
-no growth 5% NaCl (staph and micro are +)