Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
18 Cards in this Set
- Front
- Back
Supportive media |
Contain protein extract of beef or milk support growth of non fastidious organism. Staphylococcus aureus, E coli Mueller-hinton |
|
Enriched media |
Same nutrient in supportive but also contain additional supplements such as blood, serum, egg yolk, mineral. Fastidious organism. |
|
|
Selective media PEA |
Are supportive or enriched media dyes and chemicals/antibiotics added to restrict growth of some microorganisms. Preferentially select for the growth of others. |
|
|
Differential |
Are supportive or enriched. See biochemical characteristics. Growth characteristics and colony morphology. Ex lactose fermenters |
|
|
No gram wall |
Mycoplasma. Not detected by Gram stain |
|
|
Staphylococcus |
White colony, opaque, smooth |
|
Micrococcaeae |
Mustard colony |
|
Trypticase Soy Agar with 5% Sheeps Blood SBA |
Assess hemolytic properties of bacteria. |
|
Mannitol Salt Agar(MSA) |
7.5 % NaCl Carbohydrate: Mannitol Phenol red: pH indicator Neutral+ alkali: red/pink Acidic:yellow Ferment mannitol:yellow |
|
Catalase |
Break down hydrogen peroxide to water and oxygen (+) Bubbling (=) no bubbles |
|
Bacitracin susceptibility (A) |
1. Make broth 0.5 McFarland turbidity Sensitive >/equal to 10 mm Resistant>equal to 10 mm |
|
Coagulase Free coagulase |
Coagulase enzyme cause fibrin in plasma to clot. 2 types produced -Bound coagulase:exterior cell wall -Free coagulase:cell into enviroment |
|
Novobiocin susceptible (C) |
Resistant < 16 mm Sensitive > 16 mm |
|
|
2 bacteria do not stain with gram stain |
mycoplasma ureaplasma |
|
|
Why is obtaining isolated colonies important |
Biochemical testing Perform subculture |
|
|
Incubate culture of H. influenzae in Co2. Impact if not incubated |
H. influenzae anarobic. |
|
|
Gram negative(=) |
Escherichia coli Haemophilus influenza |
|
|
Gram positive(+) |
Staphylococcus aureus Enterococcus fecalis Corynebacteriun spp. Baccilus subtilis |
