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11 Cards in this Set
- Front
- Back
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how detect Ag-Ig rxns in leukocytes
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microlymphocytotoxicity
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how detect Ag-Ig rxns in platelets
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ELISA or SPRCA (solid Phase Red Cell Adhesion Assay)
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ABO Antigens determined by
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single locus on chrom 9, which codes for a glycosyltransferase (makes sense since ABO=carbs/sugar (glycosyl))
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which glycosyltransferase transfers what?
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-"A" glycosyltransferase transferse N-acetylgalactosamine
-"B" glycosyltransfersase transfers D-galactose -"O" codes for nonfuctioning protein (mutated A) |
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what is Landsteiner's law
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after 6 months old, you develop Ig to the blood groups you don't have (does not require previous exposure to foreign RBC's)
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Ig to A and B antigens are of what type
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-Ig to A and B Ag are mostly IgM, thus ABO blood type deterined by direct agglutination at room temp w/o use of antihuman globulin (no Coombs reagent)
-Individuals with Type O also have IgG component |
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what is MCC of HDN?
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ABO incompatibility
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genetic code for Rh?
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-one locus codes for presence/absence of D antigen
-second locus codes for 4 other common Ag: C, c, E, e |
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Rh+ means what?
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-presence of D antigen
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MCC of *severe* HDN?
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Rh incompatibility (erythroblastosis fetalis)
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what does a positive DAT mean?
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presence of IgG or complement (C3b and C3d) on RBC's (a positive DAT is abnl!)
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