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18 Cards in this Set
- Front
- Back
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Recommended number of specimens to examine?
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3 specimens within 10 day span, every other day if possible.
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Time limits to examine specimens?
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-within 30 minutes of passage for liquid/soft stools OR place in preservative.
-within 3-4 hours for formed stools or place aliquot in preservative and refrigerate the remainder. |
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Reject fecal specimens when:
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-contaminated with urine (destroy motile organisms), water, or oil/barium enemas, antibiotics
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What time should sputum sample be collected?
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Early moarning (most concentrated)
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Liquid fecal consistancy:
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Trophozoites
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Soft fecal consistency:
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Protozoans cysts and trophs
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Formed fecal consistency:
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Protozoan cysts
*Helminth eggs maybe found in any type of stool specimen* |
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What is refrigeration good for?
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eggs, larae, amoebic cysts
*Do NOT refrigerate if you suspect amoebic trophozoites* |
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MIF
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Merthiolate-Iodine-Formalin
concentration as well as preservation good for larvae & amoebic cysts |
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PVA
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Polyvinyl alcohol
-amoebic trophs -can prepare permanent stain slides from specimenns preserved this way. |
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SAF Preservation
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Sodium Acetate-Acetic Acid-Formalin
-amoebic trophs |
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Concentration Formalin Ethyl Acetate Sedimentation forms four layers:
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1. ethyl acetate
2. debris 3. formalin 4. sediment (contains parasites) |
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What does ethyl acetate remove?
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Fats & oils
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What is the purpose of formalin?
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Preserves organisms
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Advantages of Formalin Ethyl Acetate Sedimentation?
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easy to perform, can stay in formalin stage indefinitely
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Zinc Sulfate Flotation Technique of Concentration principle?
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Specific Gravity of zinc sulfate is greater than ova, cysts, and larvae; therefore they float on top of the zinc sulfate solution
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What is the specific gravity of zinc sulfate solution?
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1.18
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Disadvantages of zinc sulfate flotation method?
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large eggs (schistosomes) and operculated eggs (D. latum) sink quickly.
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