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52 Cards in this Set

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Define intestinal protozoa
They are unicellular organisms
Cyst: infective stage
Trophozoite: Reproductive stage
What is the classification of intestinal protozoa based on?
method of locamotion
What are the 5 classification of intestinal protozoa?
Amoebae: Psuedopods
Flagellates: Flagella
Ciliates: Cilia
Coccidia: nonmotile
Microsporidia: nonmotile
What structures are the common features in intestinal protozoa?
Nucleus: containing chromosomes: chromatin and karyosome
Cytoplasm may contain:
contractile vacuoles
RBC's
Yeast
Bacteria
Pseudopodia
Chromatoidal Bodies
Define Chromatioidal bodies
condensed RNA material
rod shaped
storage of glycogen and protein
seein in CYST FORM ONLY
What are the chromosomes found in the structure of intestinal protozoa?
Chromatin: inside nucleus wall in clumps
Karyosome: clot or mass of chromatin; central or eccentric placement
How are intestinal protozoa transmitted?
Host to host
ingestion through contamintated food/water
Define: excyst and encyst
Excyst: in small intestine
Encyst: in large intestine
What is the infective stage of intestinal protozoa?
cyst
What is the diagnostic stage of intestinal protozoa?
cyst and/or the troph seen in stool.
List the Amoeba intestinal protozoa
Entamoeba Histolytica
Entamoeba Hartmainni
Entamoeba Coli
Endolimax Nana
Iodamoeba Butschlii
What is the disease caused by Entamoeba Histolytica?
Amoebic dysentery with bloody stools
How is Entamoeba histolytica transmitted?
through fecal contaminated water supply
What does Entamoeba Histolytica secrete?
Proteolitic enzyme: Tissue invasive producing necrosis
Characteristics of E. Histolytica cyst
4 nuclie with central karyosome
fine peripheral chromatin
clean cytoplasm but sometimes has chromatoidal bodies present
Characteristics of E. Histolytica troph
single nucleus with central karyosome
fine peripheral chromatin
cytoplasm with no contracile vacuoles; finely granule
Ingested RBC's
What are the diagnostic features of the E. Histolytica troph?
Ingested RBC's
Entamoeba Hartmainni
Non pathogenic
small "variant" E. Histolytica
have no ingested RBC's
E. Hartmainni cyst and troph
Small E. histolytica
Entamoeba Coli Cyst
4-8 nuclei
large eccentric karyosome
2 nuclei in immature cyst
chromatoidal bodies
E. Coli troph
single nucleus and eccentric karysome
RBC's never present
Cytoplasm coarsely granular
Chromatin is more clumpy than smooth
Are Endolimax Nana Commensal or non commensal?
Commensal
Endolimax Nana Cyst
4 nuclei dense and stain red
small and oval
no peripheral chromatin
Endolimax Nana troph
Single nucleus with large karyosome
Are Iodamoeba Butschlii commensal or non commensal?
Commensal
Iodamoeba Butschlii cyst
single nucleus with eccentric karyosome surrounded by hyaline area
Large cytoplasmic glycogen vacuole that stains dark brown with iodine
What is the diagnostic feature of Iodamoeba Butschlii?
Large cytoplasmic glycogen vacuoles that stains dark brown with iodine
Iodamoeba Butschlii troph
single nucleus usually centrally located
nucleus surrounded by hyaline area
cytoplasm may or may not contain glycogen vacuole
What are the intestinal protozoa flagellates?
Dientamoeba Fragilis
Giardia Lamblia
Trichomonas Vaginalis
Dientamoeba Fragilis
Disease is most commen in children
seen in TROPH STAGE ONLY
2 nuclei (binucleated)
no external flagellum
pseudopodia
What is the most common parasite in the US?
Giardia Lamblia
How do you acquire Giardia Lamblia?
Ingestion of cysts
found in contaminated water
Diseases of Giardia Lamblia
Travelers Diarrhea (hikers, campers)
Diarrhea, abd pain, malabsorption
This is a reportalbe communicable disease
Giardia Lamblia cyst
oval with smooth cell wall
4 nuclei with central axostyle
parabasal body
resists chlorination
Giardia Lamblia troph
Pear shaped, bilaterally symmetrical
central axosytle with 2 nuclei
2 parabasal bodies
4 pair of flagella
"sucking disk" for intestinal aattachment
Trichomonas Vaginalis Disease
This is a sexually transmitted diease
causes vaginitis
How is Trichomonas Vaginalis Diagnosed?
By vaginal wet prep
This can be seen in urine sediment also
What is the most common parasite in the US?
Giardia Lamblia
How do you acquire Giardia Lamblia?
Ingestion of cysts
found in contaminated water
Diseases of Giardia Lamblia
Travelers Diarrhea (hikers, campers)
Diarrhea, abd pain, malabsorption
This is a reportalbe communicable disease
Giardia Lamblia cyst
oval with smooth cell wall
4 nuclei with central axostyle
parabasal body
resists chlorination
Giardia Lamblia troph
Pear shaped, bilaterally symmetrical
central axosytle with 2 nuclei
2 parabasal bodies
4 pair of flagella
"sucking disk" for intestinal aattachment
Trichomonas Vaginalis Disease
This is a sexually transmitted diease
causes vaginitis
How is Trichomonas Vaginalis Diagnosed?
By vaginal wet prep
This can be seen in urine sediment also
Trichomonas Vaginalis is found in what form?
Troph only
Trichomonas Vaginalis troph
pyriform shape (pear shaped)
2 pair of flagella
undulating membrane
define undulating membrane
to cause to move in waves
What are the intestinal protozoa cilates?
Balantidium Coli
How do you obtain Balantidium Coli?
Man ingests the cyst form through contaminated water or food
this invades bowel mucosa
not common in U.S.
This is the only ciliate that infects man
What is the only ciliate that infects man?
Balantidium Coli
Balantidium Coli cyst
Oval or spherical shaped
Macro and micronucleus present
thick refractile cell wall
cilia inside cyst wall: none on outer surface
Balantidium Coli Troph
Large, Ovoid, Covered with cilia
Kidney bean shaped macronucleus
Micronucleus present tucked in curve of macronucleus
Food and contractile vacuoles in cytoplasm