Other Other Antigens

Front 1

What type of quantitative relationship is seen for I/i?

Back 1

Reciprocal; lots of i at birth, decrases as I increases.

Front 2

How does I develop?

Back 2

Branches from i.

Front 3

What is the frequency of the i phenotype?

Back 3

very rare; 1 in 10,000

Front 4

What 3 phenotypes exist for the Ii system?

Back 4

I adult
I cord
i adult

Front 5

What type of antigen is on cells in i adult patients?

Back 5

Trace I
Much i

Front 6

What are the sources of Ii antigens?
What cells are Ii Ag's on?

Back 6

Saliva -> small amt of I
Human milk -> more I
Serum/plasma -> I and i
-On RBCs, Lymphocytes, pltlts

Front 7

Which Anti-I/i is more common?
Why?

Back 7

-Autoanti-I.
-B/c alloanti-I would be in homozygous i phenotypes, which are very rare.

Front 8

When would Autoanti-I be detected? Why?

Back 8

In the autocontrol IS; because it is IgM and reacts at cold temps.

Front 9

-What is optimum reactivity for Anti-I?
-Does it activate complement?
-Enzyme response?
-HTR/HDN?

Back 9

Yes.
Enhanced with enzymes.
Doesn't cause HTR/HDN.

Front 10

What is Autoanti-I commonly associated with? (2 things)

Back 10

1. CAIHA
2. Mycoplasma pneumonia. KNOW THIS!!!

Front 11

-What type of Ab is Anti-i?
-Optimum temp?
-HTR/HDN?

Back 11

-Naturally occuring IgM
-RT, 4'C.
-No

Front 12

What distinguishes Anti-I from Anti-i?

Back 12

Ability to react with cord cells or I- cells; Anti-i is better b/c there is much on these young cells.

Front 13

What is Anti-i associated with?

Back 13

Infectious mononucleosis
Occasional CAIHA.

Front 14

-What type of Ab is Autoanti-IH?
-Optimum temp?
-HTR/HDN?

Back 14

Naturally occuring IgM
RT, 4'C
No

Front 15

What blood type ABO usually has Anti-IH?
What cells does the Ab react best with?

Back 15

A1 individuals

Best w/ O/A2 cells with much I and H Ag to react with.

Front 16

Anti-I: what cells does it react with?

Back 16

All 4+ except Cord O

Front 17

Anti-IH: what cells does it react with?

Back 17

-A2 and O are 4+
-All others are 0-2+

Front 18

Anti-i: what cells does it react with?

Back 18

-Only O cord cells;
-All others are 0-2+

Front 19

What antigens are in the P blood group system?

Back 19

P1, P, and Pk.

Front 20

How frequent is P1 antigen?

Back 20

Varies

Front 21

What phenotypes exist for the P group?

Back 21

P1
P2
P1k
P2k
p

Front 22

P1 phenotypes have:
What antigens?
What antibodies?
How frequent in white/blacks?

Back 22

Ag: P1 and P
Ab: None
80% of whites, 95% blacks.

Front 23

P2 phenotypes have:
What antigens?
What antibodies?
How frequent in white/blacks?

Back 23

Ag: P
Ab: Anti-P1
20% of whites, 5% blacks.

Front 24

P1k and P2k phenotypes have:
What antigens?
What antibodies?
How frequent in white/blacks?

Back 24

Ag: P1, pk or just pk
Ab: Anti-P
Rare

Front 25

p phenotypes have:
What antigens?
What antibodies?
How frequent in white/blacks?

Back 25

Ag: None
Ab: Anti-P+P1+pk
Rare

Front 26

-What type of Ab is Anti-P1?
-Does it bind complement?
-What is optimum rxtn temp?

Back 26

-Naturally occuring IgM or common alloantibody in P2 pts
-Rarely
- RT or 4'C

Front 27

-How immunogenic are Anti-P1 reactions?
-Enzyme response?
-HTR/HDN?

Back 27

-Varied reactivity
-Enzymes enhance.
-Doesn't cause HDN/HTR.

Front 28

-What type of Ab is Anti-P?
-Does it bind complement?
-What is optimum rxtn temp?

Back 28

-Naturally occuring IgM;rare alloantibody in Pk1/2 pts
-May bind complement.
- RT or 4'C

Front 29

-How immunogenic are Anti-P reactions?
-Enzyme response?
-HTR/HDN?

Back 29

-VERY IMMUNOGENIC
-Enhanced
-DRAMATIC HTR, rare HDN.

Front 30

What is Anti-P associated with?

Back 30

ParoxysmalCH, IgG biphasic hemolysin.

Front 31

What is Anti-P, P1, Pk also known as?

Back 31

Tja

Front 32

What type of Ab is Tja?

Back 32

-Rare alloantibody in p phenotype patients.
-Naturally occuring
-IgM or IgG

Front 33

Does Tja bind complement?
Does it cause HTR/HDN?
What is its optimum temp?

Back 33

Yes.
Yes.
RT, 4'C, IAT. any

Front 34

what type of hemolysis does Anti-P,P1,Pk cause?

Back 34

In vitro.

Front 35

What is Tja associated with?

Back 35

Spontaneous abortions early in pregnancy.

Front 36

Ok; of the P system ab's:
-Which is most significant?
-Which is insignificant?
-Which Ag is more frequent?
-What are the building blocks?

Back 36

-P is significant
-P1 is insignificant
P1 is mre frequent than P
Paraglobosides are bblocks.

Front 37

What is unique about the Lewis system?

Back 37

Not a blood group system, because the Ags aren't made on RBCs.

Front 38

Where are the Lewis antigens made?

Back 38

In the plasma.

Front 39

What are plasma Lewis Ags?
What are saliva Lewis Ags?

Back 39

-Glycolipid
-Glycoprotein

Front 40

What genes affect Lewis Ag expression, and what type of inheritance exhibit?

Back 40

Le (FUT3) and Se (FUT2);
Le and Se --> dominant
le and se --> amorph

Front 41

What do the Le and Se genes encode?

Back 41

glycosyltransferases

Front 42

What sugars are made when Se or Le antigens expressed?

Back 42

Both = L-fucose.

Front 43

What makes Le-a unique?

Back 43

It is identical to Type 1 H chains except the Fuc is put on GlcNac instead of Gal.

Front 44

What makes Le-b unique?

Back 44

It is identical to Le-a except it has 2 Fuc's.
basically an H type-1 with an extra Fuc stuck on.

Front 45

How frequent is H type 1 antigen?

Back 45

Very frequent; 80% have it.

Front 46

What phenotypes does these genes encode?
LeSe; Le sese; lele Se (sese)

Back 46

LeSe --> Le(a-b+)
Le sese -> Le (a+b-)
sese --> Le(a-b-)

Front 47

Which of these are secretors?
LeSe; Le sese; Lele, Se; sese

Back 47

Secretors are LeSe, Lele Se

Front 48

What's the most common type of Lewis phenotypes in whites and blacks?

Back 48

Whites: Le(a-b+) 72%
Blacks: Le(a-b+) but only 50%

Front 49

What is 2nd place for Lewis phenotypes?

Back 49

Whites: Le(a+b-)
Blacks: Le(a-b-)

Front 50

How developed are the lewis antigens at birth?

Back 50

not very

Front 51

Outline lewis antigen development from birth.

Back 51

Le(a-b-), (a+b-),
(a+b+), (a-b+).

Front 52

What is weird about Lewis antigens and pregnant women?

Back 52

They go from a-b+ to a-b- and can develop an antibody.

Front 53

Why does the Lewis antigen disappear in pregnancy?

Back 53

Lipoprotein levels increase greatly, and bind all the antigen.

Front 54

What cells are the lewis antigens found on?

Back 54

Red cells
Platelets
Lymphocytes

Front 55

What antibodies do we worry about in the lewis system?

Back 55

Anti-Lea
Anti-Leb
Anti-LebH

Front 56

What patients develop
Anti-Lea?

Back 56

le --> Le(a-b-)

Front 57

-What type of Ab is Anti-Lea?
-Does it bind complement?
-What is optimum temp?

Back 57

Naturally occuring IgM; few G
Yes; it binds complement.
Temp is RT to AHG.

Front 58

How does Anti-Le(a) respond to enzymes?
Is it significant? Cause HTR or HDN?

Back 58

-Enzymes enhance it.
-Not significant if not active at Coombs.
-Rare cause of HTR, no HDN.

Front 59

Why doesn't Lea cause HDN?

Back 59

Because babies don't have developed lewis antigens.

Front 60

Who is Anti-Leb mostly seen in?

Back 60

Mostly Lea-b-, rare a+b-.

Front 61

What type of antibody is Anti-Le(b)?

Back 61

-Naturally occuring IgM; a few are IgG like anti-Lea.

Front 62

What distinguishes Anti-Leb from Anti-Lea?

Back 62

B binds complement.
Everything else is same as Lea.

Front 63

What is Anti-LebH seen in?

Back 63

Compound phenotypes
O>A2>AB>A2B>B>A1>A1B

Front 64

OK: how does an LeSe differ from an Lese?

Back 64

LeSe has less Lea in saliva than an Lese, but LeSe has Leb in saliva and Lese doesn't.

Front 65

How does leleSe differ from lelesese?

Back 65

leleSe has Tpe 1H in saliva, but lelesese only has Type 1 in saliva.