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50 Cards in this Set

  • Front
  • Back

Diffusion

Uniform distribution of solute particles in a solution, regardless of the presence of a membrane

Osmosis

Passage of water through a semi-permeable membrane as a result of concentration gradient

Hemolysis

RBC rupture; Hypotonic

Turgidity

State of being turgid or swollen

Crenation

RBC shrink; hypertonic

Plasmolysis

Plant cell membrane shrinks or may be destroyed

Plasmolysis

Plant cell membrane shrinks or may be destroyed

Head part

Hydrophilic

Causes the rapid darkening (usually browning) of fruits and vegetables after they have been peeled, slice or cut

Enzymatic activity

2 mechanisms of brown color

1. Oxygen from the air


2. From the intercellular spaces within the tissues with the cut surface that contains phenoloxidases (PPO) and phenolic compounds as suitable substrates

2 undesirable changes produced in enzymatic activity

1. Darkening of the cut surface of fruits and vegetables


2. Deterioration of flavor, odor and nutritive value

The methods of preventing most commonly practiced concentrates on the __ and __ because the elimination of the substrate (phenolic compounds) is not practical

Enzyme and oxygen

2 principals of enzymatic browning

1. Inactivation of the enzyme (PPO)


2. Minimizing contact with oxygen

3 inactivation of the enzyme (PPO)

1. Blanching


2. Use of inhibitors


3. Lowering of pH

2 minimizing contact with oxygen

1. Use of syrup


2. Use of antioxidant such as ascorbic acid and sulfur dioxide

2 procedures in enzymatic activity

1. Preparation of soaking solutions


2. Preparation of samples

5 steps in preparation of soaking solution

1. Ascorbic acid (0.5%) solution


Pulverize commercial ascorbic acid tablet. Dissolve 0.2g ascorbic acid tablet and dissolve in 40ml of hot water. Cool the solution


2. Syrup


Add 20g of sugar in 40ml of water. Heat mixture until thick mixture is formed.


3. Salt solution


Dissolve 20g of salt in 40ml water


4. Calamansi


Squeeze juice from calamansi


5. Plain water


50ml

6 steps in preparation of sample

1. Wash and dry the fruit and vegetable samples


2. Peel and/or cut (about 1cm thick) 12 slices of each sample


3. Immediately after slicing, dip two slices each into the 5 soaking solutions prepared. Make sure that both sides have been soaked. The 2 extra slices will be used for the control


4. After 1 minute of soaking, remove the slices


5. Arrange the slices on a plastic plate and label to indicate the soaking solution used in each case. Do not cover. Allow to remain exposed to air for around 45 minutes


6. Compare the appearance or the presence of surface browning in each sample

Meaning of pH

Potential hydrogen

4 reagents used in pH

1. 0.1M sodium acetate


2. 0.1M acetic acid


3. 0.1M HCl


4. 0.1M NaOH

3 indicators used in pH

1. Phenolpthalein


2. Congo red


3. Methylene blue

Used to measure pH

pH meter

Solutions that maintain a relatively constant pH when an acid or base is added

Buffer

3 components of buffer

1. Acetic acid


2. Pyridine


3. Ammonia

Is a measure of the hydrogen ion concentration of a solution

pH

Head part

Hydrophilic

Tail part

Hydrophobic

It is used to determine what ions came out from the dialyzing bag

AgNO3

Is a thin semi-permeable membrane that surrounds the cytoplasm of a cell

Cell membrane (plasma membrane)

4 compositions of a cell membrane

1. Phospholipids - a lipid of glycerol, fatty and acid tails, and a phosphate based head group


2. Cholesterol - it maintains the fluidity of cell surface membrane


3. Proteins - helps in providing the support and shape to the cell


4. Glycolipids - help cell to recognize other cells of the body

Is any biological or synthetic membrane that allows certain molecules or ions to pass through while restricting others

Semi-permeable membrane

2 transport systems in biological membrane

1. Active transport


2. Passive transport

Movement of material against a concentration gradient (low to high)

Active transport

Movement of material along a concentration gradient (high to low)

Passive transport

5 factors that promote osmosis

1. Temperature


2. Surface area


3. Difference in water potential


4. Pressure


5. Light and dark

Difference between diffusion and osmosis

Diffusion can occur in any mixture even when two solutions are separated by a semi-permeable membrane.


Osmosis exclusively occurs across a semi-permeable membrane

Requires same osmotic pressure

Isotonic

Requires higher osmotic pressure

Hypertonic

Requires lower osmotic pressure

Hypotonic

Concentration of sodium chloride

0.9%

Concentration of glucose

5%

Swelling caused by excess fluids trapped in body's tissues

Edema

Unit of molar mass

mol/L

Ideal gas content

0.0821 L*atm/mol*K

Unit of temperature

Kelvin (K)

Unit of osmotic pressure

atm

5 samples used in enzymatic browning

1. Apple


2. Banana


3. Pears


4. Eggplant


5. Potato

6 soaking solutions

1. Control


2. Ascorbic acid


3. Sugar syrup


4. Salt solution


5. Calamansi


6. Plain water

2 reasons why ascorbic acid is the most effective

1. It is an antioxidant


2. It lowers pH level

2 reasons why ascorbic acid is the most effective

1. It is an antioxidant


2. It lowers pH level