Nuclear And Cytoplasmic Staining

Front 1

During H&E staining if ammonia is incompletely removed by washing the result will be

Back 1

poor staining with eosin

Front 2

Hematein is formed when hematoxylin is subjected to the action of

Back 2

sodium iodate

Front 3

Most cytoplasmic proteins are

Back 3

acidophilic

Front 4

A tissue component that takes up a cationic dye is said to be

Back 4

basophilic

Front 5

What hematoxylin will stain the mucin in goblet cells

Back 5

Gill

Front 6

The giemsa stain is most satisfactory if the pH is between

Back 6

6.4 and 6.9

Front 7

Basic dyes have what charge

Back 7

POSITIVE i am positively basic

Front 8

While staining a rack of sections. it is noted that the water following the rehydrating alcohols turns very cloudy this can be corrected by

Back 8

changing the alcohols

Front 9

H&E sections of liver show very dark nuclei and some blue staining of the cyoplasm. this is most likely from

Back 9

inadequate differentiation of the hematoxylin

Front 10

H&E sections of SMALL intestine reveal muscle, collagen, and red blood cells all stained the same shade of pink. This indicates

Back 10

the eosin is not properly differentiated

Front 11

Water bubbles are seen microscopically on H&E sections. this can be prevented by

Back 11

making sure the dehydrating step is complete.

Front 12

H&E sections show hazy blue nuclei, but recuts from tissue processed a week previoulsy show exeleent muclear staining. This is likely due to

Back 12

the use of too much heat during processing

Front 13

A feulgen stain is requested on a section of Bouin fixed lymph node. The best course is to

Back 13

ask if there is tissue in another fixative.