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11 Cards in this Set
- Front
- Back
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what can block NT release?
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when you remove calcium
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calcium concentration in versus out
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2 mM out and 200 nM in
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what is ECa?
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+ 120 mV
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how to test if Calcium is necessary for transmitter release
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load the terminal with a fast Ca chelator such as BAPTA or EGTA, meausre intraterminal Ca with a Ca-sensing dye such as fura-2, use caged calcium (lysed by UV light)
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how many molecules of NT per vesicle?
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1000-60,000 molecules per vesicle
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synaptotagmin
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the calcium sensor for release; it binds calcium
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composition of vesicles
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~2000 molecules per vesicle; 200 protein molecules per SV
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function of synaptic vesicle proteins include
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localizing SVs within the nerve terminal (synapsins, rabs) transport across SV membrane (proton pump) dock and fusion to plasma membrane (VAMP, synaptotagmin)
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VAMP/synaptorevin
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are v-SNAREs
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SNARE proteins:
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syntaxin and SNAP-25 are t-SNAREs bind to one another and play a role in the co localization of calcium channels and sites of transmitter release
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SNARE hypothesis
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the selective docking of a transport vesicle with appropriate target membrane occurs through the formation of a complex between a vesicle membrane protein (v-SNARE) and target membrane protein (t-SNARE)
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