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5 Cards in this Set
- Front
- Back
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Cell Sorter/Flow Cytometry
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-Direct Enumeration
-Utilize dyes which stain cells -Single cell at a time - +'s = Mechanical (no human error) & Low eye strain - -'s = Costly & only good for fluid samples |
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Direct Microscopic Count
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-Direct Enumeration
-Area of Field must be determined -Shows both viable and dead -Lots of counting and human error -Quick and easy to use with raw milk |
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Pour plate
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-Indirect method
-45 degrees C agar (if too hot then can kill organisms) |
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TTC
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Colorless die until metabolized (turns red)
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Spread plate
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-Indirect method
-Pre-hardened medium poured into plates -Heat is not an issue -Good for strict aerobes -May get spreaders = spread across entire plate because not absorbed |
