Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
15 Cards in this Set
- Front
- Back
|
What is the maximum resolution of any optical light system? |
200 nm |
|
|
What are the 2 types of aberrations and why do they occur? |
Spherical aberrations occur due to the curvature of the lens along the extreme. Chromatic aberrations occur due to the different focal lengths of different colour of light. |
|
|
What does the field aperture do? What does the condenser aperture do? |
The condenser aperture controls the brightness, the field controls the area of sample illumination
|
|
|
What light are we looking at in bright field imaging? What light are we looking at in dark field |
Bright Field = Undiffracted Light DarkField = Diffracted Light |
|
|
What is the general relationship between depth of field and resolution |
Depth of Field = 1/Resolution |
|
|
What does DIC stand for? What type of crystal is used? What is it used for? |
Differential Interference Contrast. Birefringent Crystal It determines the DERIVATIVE of the height across the sample. |
|
|
What does PLM stand for? What does it allow us to determine. |
Polarized Light Microscopy. Allows us to determine the phase change of light of the sample. |
|
|
What types of light does phase contrast microscopy look at? What types of samples is it best for? |
Looks at undiffracted and diffracted light. This is best for transparent samples. |
|
|
What does a dichroic mirror do? How is it used in fluorescence microscopy? |
A dichroic mirror reflects high energy light and allows low energy light to pass through. We use it to reflect a beam to initiate fluorescence in our sample. The fluoresced light will be low enough wavelength to pass through to the observer. |
|
|
How does a confocal microscope scan over every point on the sample. |
2 moving mirrors allow us to direct our light to a specific spot. |
|
|
If our sample doesn't fluoresce naturally, how can we use confocal microscopy. |
Dye the sample with fluorescent material |
|
|
How do we guarantee we are only observing a single point in confocal microscopy? |
We use a pinhole to limit our light. This ensures a 1:1 mapping |
|
|
How can we manipulate a Bright Field Image to look like a Dark Field |
Invert the colour scheme |
|
|
A material that is anisotropic is _____________ |
a material that creates a phase difference |
|
|
Does photo luminescence rely on a direct or indirect band gap |
Indirect Band Gap |
