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18 Cards in this Set
- Front
- Back
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characteristics |
smallest of the small <1 micron lack cell wall |
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mycoplasma cultures |
media must contain serum and yeast extract can add penicillin to inhibit other bacteria |
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incubation |
increased CO2 up to 1 month colonies are too small to be seen without the naked eye--> stereoscopic microscope |
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mycoplasma ID |
don't take up gram stain so used Dienes stain short blue bacilli, look pleomorphic immunological ID Molecular ID |
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Mycoplasma pneumoniae |
primary atypical pneumonia walking pneumonia malaise, cough with mucoid sputum lasts 2-3 wks in young adults causes up to 50% community acquired pneumonia |
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treatment of M. pneumoniae |
erythromycin |
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Mycoplasma pneumoniae ID |
cold agglutinins: IgM immunologicL EIA for IgG or IgM molecular: NAAT tests (Nucleic Acid Amplification Tests)
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Genital Tract Mycoplasmas |
Mycoplasma hominins: postpartum infections in women with ruptured membranes Mycoplasma genitalium: non-gonococcal urethritist, can cause cervicitis |
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Mycoplasma as Normal Flora |
Mycoplasma fermentans: genitourinary tract, oropharynx Mycoplasma salivarium & orale: oropharynx |
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L Forms |
any bacteria, become cell wall deficient with high concentrations of Penicillin and NaCl |
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Streptobacillus moniliformis |
easily reverts to cell wall deficient form causes rat bite fever |
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Legionella pneumophilia |
common in water lung loving bacteria |
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Legionnaire's Disease |
always in water systms transmission: inhalation.aspiration of water, not from person common in middle-ages male smokers can kill is water is heared to > 70 C for 72 hrs or increase chlorine |
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Legionnaire's Disease Symptoms |
starts with flu'like symptoms 1 day later: fever up to 105, chills, non-productive cough 2-3 days later: lobar pneumonia
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treatment of Legionnaire's Disease |
Erythromycin Erythromycin plus rifampin |
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Pontiac Fever |
antigenic variation of Legionella pneuophila mild, self-limiting |
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Legionella pneumophila Lab DX culture |
soc: sputum culture is gold standard but lacks sensitivity requires cysteine for growth buffered charcoal yeast extract media (BCYE) or BCYE with cysteine usually takes 3-4 days to grow, 35 C colonies have ground glass appearance |
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Legionella pneumophila Lab DX tests |
tiny gnb, hard to stain, need nethanol weak oxidase +, catalase + Direct or Indirect fluorescent antibody PCR Urine antigen test: pt can secret antigen months after even having infection |
