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23 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Source of restriction endonuclease |
Bacteria |
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Function of restriction endonuclease enzymes |
Used extensively in recombinant DNA technology to cut DNA into smaller fragments
This enzyme is widespread in ______ |
Used ______ in ______ ____ ____ to ____ ______ into ______ ______
Bacteria |
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Function of restriction endonuclease enzymes |
Used extensively in recombinant DNA technology to cut DNA into smaller fragments |
_______________ in _____________________ to ___________ into __________ |
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Function of restriction endonuclease enzymes |
Used extensively in recombinant DNA technology to cut DNA into smaller fragments
Mechanism? |
Recognize short specific nucleotide sequences (usually palindromes) and cut DNA at those site |
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Mechanism of restriction endonuclease enzymes |
_______ ____ _____ ____ _____ (____ _____) and ______ ____ at _____ _____ |
Recognize short specific nucleotide sequences (usually palindomes) cut DNA those site |
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Mechanism of restriction endonuclease enzymes |
Recognize short specific nucleotide sequences (usually palindromes) and cut DNA at those site |
_________________________________________ (____________) and _______________ at ________ |
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What does endonuclease enzymes usually recognize? |
Palindromes |
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Review Restriction endonuclease |
Bacteria |
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Examples of restriction endonuclease
EcoRI Species |
E.coli
Notes |
Recognize a particular gene |
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Examples of restriction endonuclease
BamHI
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Bacillus amyloliquefaciens |
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Examples of restriction endonuclease
EcoRI |
E.coli |
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Examples of restriction endonuclease
HaeIII |
Haemophilus aegyptius |
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Examples of restriction endonuclease
HindIII |
Haemopilus influenza |
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Examples of restriction endonuclease
Notl |
Nocardia otitidis - cavarium |
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Examples of restriction endonuclease
Sau3A |
Staphylococcus aureus 3A |
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Examples of restriction endonuclease
Stsll |
Streptomyces standford |
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Examples of restriction endonuclease
Haemophilus influenza |
Hind III |
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Examples of restriction endonuclease
E.coli |
EcoRI |
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Examples of restriction endonuclease
Haemophilus aegyptius |
HaeIII |
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Examples of restriction endonuclease
Bacillus amyloliquefaciens |
BamHI |
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Examples of restriction endonuclease
Nocardia otitidis - cavarium |
Notl |
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Examples of restriction endonuclease
Streptomyces standford |
Stsll |
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Examples of restriction endonuclease
Staphyloccus aureus 3A |
Sau3A |
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