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20 Cards in this Set
- Front
- Back
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1. Which symbol below denotes a mouse gene symbol?
a. Apc b. APC c. APOA1 d. APOA1 |
1. a, mouse gene symbols are written in italics with the first letter capitalized followed by lower case letters. Human proteins are also written in italics, but are written in all caps.
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2. Which symbol denotes a mouse protein symbol?
a. Apc b. APC c. APOA1 d. APOA1 |
2. b, mouse proteins are written using all capital letters and no italics (so are human proteins)
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3. Which symbol denotes a mouse with a recessive allele?
a. ApcMin b. Apcmin c. Apcmin d. APCmin |
3. b, alleles are written in italics and are superscripted to the gene symbol. The first letter of the dominant alleles will be capitalized, the first letter of the recessive allele will be lower case.
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4. This is a strain formed by backcrossing a locus of interest onto an inbred strain for five to nine generations (between N6 and N9)
a. Congenic b. F1 Hybrid c. Incipient Congenic d. Coisogenic |
4. C, a congenic would be for 10 or more backcrosses (greater or equal to N10)
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5. The “ : ” is used to denote what kind of strain/stock (ex. Hsd:ICR)
a. Inbred b. Conplastic c. Outbred d. Advanced intercross |
C
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6. Type of strain where a particular allele or mutation is maintained in a heterozygous state
a. Conplastic b. Consomic c. Advanced intercross d. Segregating inbred |
6. d, a good example of this is the nude mouse where the females (nu/+) are mated to (nu/nu) males
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7. Type of strain where the nuclear genome from one strain has been crossed onto the cytoplasm of another
a. Conplastic b. Consomic c. Advanced intercross d. Coisogenic |
A
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8. The “ X ” symbol is used to denote what type of cross (ex. AXB)
a. Outbred b. Recombinant inbred c. Advanced intercross d. Consomic |
B
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9. When maintaining an inbred strain, if you choose to do parent/offspring mates, then you must use which parent?
a. The younger parent b. The older parent c. It doesn’t matter |
A
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10. In the transgenic nomenclature, which part denotes the mode of insertion: Tgx(YYYYYY)#######Zzz
a. Tg b. x c. (YYYYYYY) d. ######## e. Zzz |
B
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11. Mouse chromosomes are all:
a. Metacentric b. Arcocentric c. Telocentric d. Submetacentric |
11. c, rats have both telocentric and metacentric chromosomes
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12. How many chromosomes do mice have?
a. 40 b. 42 c. 44 d. 64 |
12. a, they have 2o pairs. Some wild mice have been found to have less due to fusion of their chromosomes (Robertsonian translocation)
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13. What type of mouse is created if DNA is inserted into an ES cell, where it undergoes homologous recombination, and gene expression is disrupted. The cells are then injected into mouse blastocysts to create chimeras
a. Transgenic b. Knockout c. Knockin d. Target mutation |
13. B
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14. This strain is identical at more than 99% of genetic loci
a. Coisogenic b. Isogenic c. Congenic d. Inbred |
14. B
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15. This transplantation is made between individuals that have the same genotype (ex, mice of inbred strains)
a. Allogeneic b. Xenogenic c. Autogenic d. Syngeneic |
15. D
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16. This transplantation is made between individuals or tissues that are of the same species but antigenically distinct
a. Allogeneic b. Xenogenic c. Autogenic d. Syngeneic |
16. A
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17. If an outbred stock had been fostered, what letter(s) would be used to indicated this?
a. f b. fh c. fo d. fos |
17. a, (Crl:CD-1(SD)f ; fh stands for fostered on hand reared
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18. This is an example of what type of strain: C57BL/6J-YAKR
a. Congenic b. Recombinant inbred c. Consomic d. Coisogenic |
18. c, HOST STRAIN-Chr#DONER STRAIN
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19. This is an example of what type of strain: C57BL/6J-cph
a. Congenic b. Recombinant inbred c. Consomic d. Coisogenic |
19. D
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20. When creating a transgenic mouse by random insertion, the DNA is usually microinjected into where?
a. The female pronuclei b. The male pronuclei c. The nucleus d. None of the above |
20. B
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