Mouse Blue Book, Vol I - Chp 8

Front 1

Which of the following was the first known inbred strain of mouse, developed by C. C. Little?
a. BALB/c
b. DBA
c. NZW
d. C3H

Back 1

b. DBA

Front 2

True or False: The primary purpose of an effective genetic monitoring program is to detect genetic contamination, spontaneous mutations and genetic drift of inbred strains of mice.

Back 2

False; A genetic monitoring program will not detect rare spontaneous mutations or genetic drift.

Front 3

________ strains are produced by repeated backcrossing of a whole chromosome such as the X or Y chromosome onto an inbred strain.

Back 3

Consomic

Front 4

Researchers must be vigilant for potential genetic errors or contamination when obtaining inbred mouse strains from which of the following?
a. Their centralized institutional breeding facilities
b. Commercial breeders
c. Collaborating investigators from other institutions
d. a and c
e. All of the above

Back 4

e. All of the above

Front 5

True or False: Genetic contamination may have a significant impact on an individual biomedical researcher in terms of lost time, materials, reputation and reproducibility of results but would have little impact on the scientific community at large.

Back 5

False

Front 6

The 2 components of a genetic quality control program are a) a formal regular monitoring program to assure detection of genetic contamination and b) basic _____ ____ __________ that reduce the likelihood of contamination.

Back 6

mouse room procedures

Front 7

Which of the following is NOT a recommended measure for reducing the risk of genetic contamination?
a. Placing strains of the same coat color in different production rooms
b. Use of color-coded strain specific cage cards
c. Escapee mice found by animal technicians should be identified, as best as possible, before returning them to the breeding colony
d. Animal technicians should be trained to appreciate the importance of maintaining genetic integrity in inbred strains of mice

Back 7

c. Escapee mice found by animal technicians should be identified as best as possible before returning them to the breeding colony (Escapee mice found by the animal technicians should be euthanized at once)

Front 8

A sound genetic monitoring system should use assays that are sufficiently ___________ among strains to detect contamination in any strain in the breeding facility.

Back 8

polymorphic

Front 9

True or False: A genetic quality control program should be designed to assure the genetic integrity of mice in the breeding facility with primary attention to those animals destined for immediate distribution to biomedical researchers.

Back 9

False; A genetic quality control program should be designed to assure the genetic integrity of mice in the breeding facility with primary attention to foundation colonies or the top of the expansion pyramid.

Front 10

The theory behind the use of electrophoresis to identify differences among isoenzymes is based on mutational changes in DNA which can alter the amino acid sequences of a specific gene. This, in turn, may alter the electrical charge and therefore the electrophoretic ________ of the protein through a porous substrate.

Back 10

mobility

Front 11

Name the 2 basic methods for demonstrating alloantibodies in immunological assays.

Back 11

Hemagglutination assay and cytotoxic tests

Front 12

True or False: Mouse erythrocytes do not readily agglutinate in the presence of alloantisera diluted in a saline medium.

Back 12

True

Front 13

True or False: One advantage to serologic immunologic assays is that a positive reaction to one antiserum is specific for that H-2 type and uniquely identifies a particular mouse strain.

Back 13

13. False; One must rule out positive reactions with other types of antisera and the existence of cross of H-2 types.

Front 14

The current genetic monitoring program at The Jackson Laboratories has shifted from biochemical and immunological testing to the exclusive use of ______ __________ _____________.

Back 14

single nucleotide polymorphisms

Front 15

True or False: For practical purposes insertion or deletion of one or a few nucleotides can be considered as single nucleotide polymorphisms (SNPs)?

Back 15

True

Front 16

Which of the following statements is most true regarding single nucleotide polymorphisms?
a. Primary protein structure may be affected if the change occurs in regulatory regions.
b. Changes in the coding sequence may result in altered gene expression.
c. Although they occur with relative frequency, less than 10,000 SNPs have been identified in mice.
d. Many SNPs occur in introns and intergenic regions and have no known phenotypic effect.

Back 16

d. Many SNPs occur in introns and intergenic regions and have no known phenotypic effect.

Front 17

A normal karyotype of Mus musculus contains __ pairs of chromosomes.

Back 17

20

Front 18

Which of the following are advantages provided by SNP genotyping over other methods?
a. The abundance of SNPs in the genome allow the selection of new markers and the development of assays when necessary
b. Animals can be tested prior to breeding
c. Although testing may be time consuming, assays can be run at low cost.
d. a and b only
e. All of the above

Back 18

d. a and b only

Front 19

Regardless of the testing method(s) used, a genetic monitoring program is of no value without an accurate ______-_______ ______ to allow tracking of mice.

Back 19

record-keeping system

Front 20

True or False: Within the production colony, improvements in breeding performance or an increase in litter size of an inbred strain may be an indication of genetic contamination.

Back 20

True