Mouse Blue Book Vol I - Chp 5

Front 1

Rules for mouse nomenclatures are set by the __________________.

Back 1

International Committee on Standardized Nomenclature in Mice

Front 2

True or False. All laboratory mouse strains have genetic contributions from Mus musculus only

Back 2

False

Front 3

Define inbred strain.

Back 3

Strains maintained by brother X sister matings for 20 or more consecutive generation, and individuals of the strain can be traced to a single ancestral pair at the 20th or subsequent generation.

Front 4

An example of an inbred strain would be all of the following except:
a. CBA/HeH
b. C57BL/6J
c. SWR
d. CcS RC
e. HRS/J

Back 4

D

Front 5

If inbred strains genetically diverge with time, they are categorized as ____________.

Back 5

substrains

Front 6

Define F1 hybrid.

Back 6

Mice that are the progeny of mice of two inbred strains and are genetically identical if the female parent is from strain A and the male from strain B.

Front 7

List three commonly used outbred stocks.

Back 7

Crl:CD-1, Crl:ICR, Crl: Swiss Webster

Front 8

True or False. No two individuals from an outbred stock are the same.

Back 8

True

Front 9

What is the primary goal of an outbred stock?
a. To maintain maximum heterozygosity
b. To maintain maximum homozygosity
c. To maintain minimum heterozygosity
d. To maintain minimum homozygosity

Back 9

A

Front 10

True or False. F1 hybrid designations should always have the maternal strain listed first.

Back 10

True

Front 11

Which strain designation would C3H/HeH be under?
a. Inbred strain
b. Hybrid strain
c. Outbred strain

Back 11

A

Front 12

Discuss the rule for hybrid strain designation.

Back 12

Maternal strain X male strain in parenthesis, followed by F1

Front 13

B is the abbreviation for which hybrid strain:
a. BALB/c
b. C57BL/6 substrains
c. C57BL substrains
d. DBA/1

Back 13

C

Front 14

To name outbreds_____________________________________________.

Back 14

Use the Laboratory Code of the institution holding the stock, followed by a colon and the common rood stock name for that outbred.

Front 15

What is the Lab Code?

Back 15

Laboratory Registration Code is usually two to four letters and identifies a particular institute, laboratory, or investigator. They are also used in naming chromosomal aberrations and genetically engineered mutations. They are assigned by MGD or ILAR.

Front 16

Recombinant inbred strains are formed by crossing animals of ___________ inbred strains, followed by ________________ or more consecutive generations of ____________________ matings.

Back 16

two, 20, brother X sister

Front 17

How are recombinant inbred strains designated?
a. Uppercase abbreviation of the two strain
b. Uppercase two letter abbreviation of maternal strain name
c. Uppercase one or two letter abbreviation of maternal strain name
d. Uppercase one or two letter abbreviation of both parental strain names.

Back 17

D

Front 18

All of the following are examples of recombinant inbred strains except:
a. 129P2
b. BXD1
c. CcS3
d. AXB19/Pgn

Back 18

C

Front 19

B6D2-Sox18Ra-Op/J is an example of which strain:
a. Recombinant inbred
b. Mixed inbred
c. Mixed Noninbred
d. Congenic

Back 19

C

Front 20

Provide a description of the following strain CcS1, CcS2, CcS3.

Back 20

Three recombinant congenic strains from the CcS RC strain set, where BALB/c was the recipient strain and STS/A the donor.

Front 21

The purpose of the advanced intercross lines is to ____________________________________________.

Back 21

Increase the possibility of tightly linked genes recombining.

Front 22

Define conisogenic strains.

Back 22

Inbred strains that differ at only a single locus through mutation occurring in that strain.

Front 23

Congenic strains
a. Are produced by repeated back crosses to an inbred strain
b. Have a minimum of 10 backcross generations to the background strain
c. Designated by the full symbol of the recipient strain separated by a period from an abbreviated symbol of the donor strain.
d. All of the above

Back 23

D

Front 24

True or False. Consomic strains are inbred strains in which a particular allele or mutation is selectively maintained in herterozygous state.

Back 24

False

Front 25

Gene names should
a. always begin with an uppercase letter
b. use American or British spelling
c. be specific and brief
d. include the word mouse

Back 25

C

Front 26

True or False. Gene symbols should include tissue specificity or molecular weight designations.

Back 26

False

Front 27

Give an example of a gene name and symbol.
.

Back 27

claudin 1 Cldn1; growth hormone receptor Ghr

Front 28

Provide an example of a genetic marker that is not a gene.

Back 28

Anonymous DNA segments (D8Byu2)

Front 29

Which of the following is not true?
a. When a gene is know only by a mutant phenotype, the gene is given the name and symbol of the first identified mutation at that locus
b. Phenotypic alleles (when the underlying gene is known) are designated as superscripts of the gene symbol.
c. Normal wild-type allele of a gene is indicated by a “-“superscript to the mutant symbol or to the structural gene symbol

Back 29

C

Front 30

Define transgene

Back 30

Any DNA that has been stably introduced into the germ-line of mice or rats

Front 31

Describe the following symbol Tg(Drd1a-cre)1Gsc.

Back 31

Cre transgene with a Drd1a promoter, the first from the laboratory of Gunther Schutz (Gsc)

Front 32

Chromosome anomaly and variation symbols are/are not italicized in publications, gene symbols are/are not.

Back 32

are not, are