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16 Cards in this Set
- Front
- Back
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When run on the gel what does it mean if something is acidic or basic?
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Acidic is negative
Basic is positive |
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What is found in anion exchange column>?
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cations
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What happens in nucleic acid hybridization?
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Press filter paper againsy gel and helps transfer DNA to nitrocelluolose (allows moisture through, but not DNA) also wants negative charge
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What is an autoradiogram?
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will only show radioactive
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What is a northern blot?
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Uses RNA
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What is a western blot?
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labeled antibodie, protein on gel
2nd antibody is detectable |
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Why are legs bent?
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because of double bodns
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How are things moving in the cell?
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Move laterally in constant motion, nothin g is static in the cell
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What is a good example of carbs on the surface?
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A,B,O antigens
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Where is CFTR found?
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in epithilial lining of lungs
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Why do you need low calcium?
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Ca+ makes phosphate precipate in cell
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What can you with the xenopus egg?
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Inject mRNA for a sequence and find it with the thing
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What are the different sections of the tail/ball thing?
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11-phobic, remove closes more slowly
12-20-philic same result 21-37-delete any of thse you get a channel that closes more quickly 4. Remove too many channel no longer close %. Insert more into 21-37 closes more slowly Delete first 20 can't close Inject a peptide corresponding to the ball and it will close Ball may have charge attraction |
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What is the basal lamina?
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Holds epithilal sheet together
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What do fish do as they migrate?
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Vary lipid concentration as they migrate, don't want too fluid or too stiff
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What are rbs lacking?
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No internal membranes, nucelus, no organelles
Have cyctoskeleton Have large surface area |
