Mole Bio

Front 1

alpha 1

Back 1

uses q

Front 2

alpha 2

Back 2

i

Front 3

beta 1

Back 3

s

Front 4

beta 2

Back 4

s

Front 5

M1

Back 5

q

Front 6

M2

Back 6

i

Front 7

M3

Back 7

q

Front 8

D1

Back 8

s

Front 9

D2

Back 9

i

Front 10

H1

Back 10

q

Front 11

H2

Back 11

s

Front 12

V1

Back 12

q

Front 13

V2

Back 13

s

Front 14

fxn V1

Back 14

vascular sm m cxn

Front 15

fxn V2

Back 15

increase H20 perm and reabsorption in collecting tubules in kidney

Front 16

fxn H1

Back 16

mucus production (nasal and bronchial), cxn bronchioles, pruritis, pain

Front 17

fxn D1

Back 17

relaxes renal vascular sm m

Front 18

fxn D2

Back 18

modulates transmitter release, esp in brain

Front 19

fxn M3

Back 19

incr exocrine gland sxns

Front 20

fxn M2

Back 20

decr HR

Front 21

fxn M1

Back 21

CNS

Front 22

fxn alpha 1

Back 22

vascular sm m cxn GI, sphincter constriction, mydriasis, ejac,

Front 23

fxn alpha 2

Back 23

decr symp outflow, decr insulin, decr aq humor

Front 24

fxn beta 1

Back 24

incr HR, contractility, incr aq humor, incr renin, incr lipolysis

Front 25

fxn beta 2

Back 25

vasodilation BF to organs and mscls, bronchodilation, relax GI, incr glucagon, epp + NE rel

Front 26

Gq path

Back 26

phospholipase C converts lipids to PIP2
1) IP3->incr Ca internally
2) DAG->protein kinase C

Front 27

Gs path

Back 27

adenylcyclase convert ATP to cAMP->protein kinase A

Front 28

Gi path

Back 28

adenylcyclase decr cAMP->decr protein kinase A

Front 29

which R use Gi

Back 29

alpha 2, M2, D2

Front 30

which R use Gs

Back 30

beta 1,2, D1, H2, V2

Front 31

which R use Gq

Back 31

alpha 1, M1, H1, V1, M3

Front 32

what need for nuclear transport?

Back 32

NLS, NES

Front 33

what need for ER

Back 33

SRP

Front 34

specific fxns of ER

Back 34

-N link glycosyl
-folding by chaperones
-disulfide
-GPI anchor
KDEL=ER retention

Front 35

what's signal for ER

Back 35

KDEL means ER retention

Front 36

fxn golgi

Back 36

O link glyc and trim N link, if lyso add mannose6P

Front 37

where syn of tg occur

Back 37

in SER

Front 38

golgi's role

Back 38

protein sorting and packaging

Front 39

getting into mito

Back 39

uses TOM/TIM

Front 40

Progressive Opthal

Back 40

ANT to import ATP, mito poly delta, twinkle helicase

Front 41

MELAS

Back 41

aminoacyl of Lys nec to import (assoc w bipolar)

Front 42

MNGIE

Back 42

thymidphosphorylase to make nucleotide, resents with diarrhea/constipation

Front 43

name 3 mito dz

Back 43

Progress Ophthal, MNGIE, MELAS

Front 44

what 3 things happen to start transcript

Back 44

demethyl Histones, acetyl histones, demeth promoter

Front 45

competit agonist: change Km

Back 45

increases Km (need more substrate to compete)

Front 46

competit agonist: Vmax

Back 46

same

Front 47

noncompetit agonist changes in Km, Vmax

Back 47

Vmax lower, Km same

Front 48

on plot, how do competit and noncompetit lines appear

Back 48

competit=increase slope same x intercept (Vmax same)
noncompetit=increase x intercept, y intercept same (increasing slope)

Front 49

is Km related to binding affinity?

Back 49

no, to speed

Front 50

exn use to plot enzyme rxn

Back 50

1/v= 1/vmax + Km[S]/(Km + [S])

Front 51

describe forms DNA when see them

Back 51

A=R 10bp/turn, most DNA
B=11bp in RNA:DNA, dsRNA, or dehydration
Z=L hand turn

Front 52

genomic library

Back 52

has introns, nontranscribed regions, promoter

Front 53

if wanted to make protein, and have the sequence stored, what use to make it from

Back 53

cDNA (can use polyT to get mRNA

Front 54

cDNA

Back 54

no introns, only the sequence that codes mRNA

Front 55

describe how, where splicing occurs

Back 55

in splicesomes by snRNPs

Front 56

describe difft choices vectors

Back 56

plasmid=5kb
phage=20kb
YAC=1000kb

Front 57

role peroxisome

Back 57

detox
b oxidation LCFA
syn: cholesterol, plasmalogen (myelin) and bile acid

Front 58

name peroxisomal dz

Back 58

Zelweger (empty perox)
ALO (defect transport LCFA)
Phytanic acid problem

Front 59

Phytanic acid problem presents with? tx?

Back 59

decr night vision, tx by diet

Front 60

problem in Zelweger

Back 60

unable to import into peroxisomes

Front 61

ALO (peroxisome dz) presents with what symptoms? what's the problem?

Back 61

(peroxisome dz) neuro symptoms bc fa degradation myelin,
problem is transport LCFA

Front 62

peroxisome have mem?

Back 62

yes, 1 mem

Front 63

how peroxisomes import? what do they need to import?

Back 63

PEX5, need to import all proteins bc has none of its own DNA

Front 64

role lysosomes

Back 64

intracellular digest

Front 65

what cell has lots and lots of lysosomes

Back 65

phagocyte

Front 66

if lysosomes leak what happens?

Back 66

nothing bc the lysosomes are very acidic and the lysosome enz only work at acidic pH, not cell pH

Front 67

lipofucsion

Back 67

residual bodies after phago

Front 68

is proteasome mem bound

Back 68

nom soluble

Front 69

role of proteasome

Back 69

degrade protein labeled with Ubq

Front 70

lipid anchored proteins on C face mem

Back 70

fa, isoprenoid

Front 71

lipid anchored proteins on E face

Back 71

GPI

Front 72

O linked gly occurs

Back 72

golgi

Front 73

T/F disulfide bonds formed in ER

Back 73

T

Front 74

what can use passive transport

Back 74

small nonpolar like O2,CO2
hydrophobic-benzene
small polar uncharg ETOH, H2O

Front 75

T/F thymidine dimers are across strands

Back 75

F SAME STRAND

Front 76

excision repair defect in xeroderma pigmentosum

Back 76

uvr ABC endonuclease (can't repair thymidine dimers after exposure UV light)

Front 77

alpha amanitin does what?

Back 77

inhibits RNA poly II

Front 78

describe initiation translation

Back 78

RNA pol II opens DNA at promoter site (AT rich upstream seq, TATA, CAAT)

Front 79

name types RNA poly

Back 79

I makes rRNA
II makes mRNA
III makes tRNA

Front 80

largest RNA? most abundant? smallest?

Back 80

largest=mRNA
abundant=rRNA
smallest=tRNA
Massive, Rampant, Tiny

Front 81

what charges tRNA

Back 81

aminoacyl-tRNA synthetase, using ATP

Front 82

all tRNA's have what seq

Back 82

CCA at 3 end where aa is linked

Front 83

describe ribosome steps

Back 83

met sits in P site, incoming aa comes into A site,
hydrolyzes Met bond to tRNA, forms Met-aa bond and transfers that to E site
A=aminoacyl
P=peptidyl
E=

Front 84

is GTP or ATP used for charging aa? for translocation?

Back 84

ATP for activation
GTP for gripping and going places

Front 85

if tRNA bond is incorrect what happens?

Back 85

synthetase hydrolyzes

Front 86

DNA poly used in prokaryo

Back 86

DNA poly III elongates 5-3 and proofreads with 3-5 exonuclease,
DNA poly I excises RNA primer with 5-3 exonuclease

Front 87

how many origins of replication in bac? virus? plasmid?

Back 87

all 1, euk only with mutliple

Front 88

describe single strand DNA repair

Back 88

glycolase reognizes and removes damaged base, endo nuclease makes a break, exonuclease removes several neighboring bases, DNA poly fills in gap and DNA ligase seals

Front 89

Bloom's syndrome

Back 89

can't repair DNA s/p radiation

Front 90

ataxia telangiectasia has repair problem with what

Back 90

xrays

Front 91

Fanconi's anemia

Back 91

defect in DNA repair from cross-linking agents

Front 92

name DNA repair dz

Back 92

Xeroderma Pigment (UV light)
atxia-telangiectasia (x-ray)
Bloom's (radiation)
Fanconi's anemia (cross linking agents)