Module3 Lectures 15

Front 1

what does bone marrow consist of?

Back 1

Stoma- connective tissue
Specialized Cappilaries(sinusoidal/discontiuous)
Non hematopoeitic cells (stromal cells)

Front 2

What do stromal cells secrete?

Back 2

Steel factor

Front 3

put these in order of basic to specific cells, progenitor, mature, MHSC, precurser, PHSC

Back 3

PHSC, MHSC, Progenitor, precurser, mature

Front 4

Name 2 stem cells function

Back 4

1. self renewing
2. give rise to progenitor cells

Front 5

what is a totipotent?

Back 5

a blastomere that can turn into any type of cell

Front 6

What are the 3 names of pluripotent

Back 6

Hemocytoblast, PHSC, pluripotent

Front 7

What is another name for multipotent

Back 7

MHSC

Front 8

What does a hemocytoblast (PHSC) produce as two daughter cells

Back 8

Hemocytoblast and MHSC

Front 9

name the 2 types of MHSC

Back 9

CFU-LY: for lymphocytes- forms T and B cells
CFU-S: in spleen, for all other blood lines

Front 10

What does a progentitor cell give rise too

Back 10

Precurser cell

Front 11

What is important about precursor cells

Back 11

they are morphologically recognizable as belonging to a specific cell. ie, they have a destiny

Front 12

What is required for PHSC and MHSC to undergo mitosis

Back 12

Steel factor

Front 13

What 3 factors can stimulate CFU's to produce more of their line?

Back 13

Growth factors, colony stimulating factors or hematopoietins

Front 14

What controls the release of mature blood cells from the marrow to the blood stream

Back 14

release factor

Front 15

What is granulocytopoiesis

Back 15

the formation of granulocytes

Front 16

what stimulates granulocytopoises

Back 16

the loss of granulocytes

Front 17

name 3 characteristics that occur during granulocytopoiesis

Back 17

1. progressive reduction in cell size
2. increasing indentation and lobulation of the nucleus
3. progressive accumulation of specific granules.

Front 18

name the 4 stages of granulocytopoiesis

Back 18

1. myelocyte
2. metamylocyte
3. band cells (stab cells)
4. Mature granulocytes

Front 19

describe myelocyte

Back 19

slight reduction of cell size.
round or oval nucleus
appearance of specific granules

Front 20

describe metamylocyte

Back 20

nucleus is kidney bean shaped
specific granules present
no longer mitotic

Front 21

describe band cells

Back 21

nucleus is C or horseshoe shaped.
specific granules are present
low percentage is found in peripheral blood

Front 22

what is erthropoiesis

Back 22

production of red blood cells

Front 23

what is the biggest stimulator for erthopoiesis

Back 23

tissue hypoxia (low O2 levels)

Front 24

What are the essential cofactors for erthropoiesis

Back 24

iron, folic acid, vitamin B-12

Front 25

name the 4 general charateristics of erythropoisis

Back 25

1. decrease cell volume
2. loss of nucleus
3. loss of cytoplasmic organelles
4. increase in hemoglobin

Front 26

describe the histological color process of erythopoisis

Back 26

Blue to light blue to blue + pink to pink

Front 27

Name the stages of erthopoiesis

Back 27

basophilic erythoblasts
polychromatophilic erythroblasts
orthochromatophilic erythoblasts
reticulocyte
Mature erythocyte

Front 28

list percentage of blood layers seperated by certerfugeation

Back 28

Hematocrite 40-45%
buffy coat 1%
plasma 55%

Front 29

list the plasma protiens

Back 29

albumen
globulins
clotting protiens
complement protiens
plasma lipoprotiens

Front 30

what are the two types of romanousky stains

Back 30

methylene blue and eosin
wright-giemsa

Front 31

what stain do you use for reticulocytes

Back 31

supravital and cresyl blue

Front 32

the 2 WBC groups ( granulocytes and agranulocytes) are based on what 2 factors

Back 32

Shape of nuclei
presence/ absence of granules

Front 33

do granulocytes have lobed or rounded nucleus

Back 33

lobed

Front 34

name the 3 WBC granulocytes

Back 34

neutrophils
eosinophils
basophils

Front 35

name the 2 WBC agranulocytes

Back 35

monocytes
lymphocytes

Front 36

give 2 other names for PMNs

Back 36

neutrophils and polys

Front 37

what is the size and percentage of WBC count of neutrophils

Back 37

12-14 and 60-70%

Front 38

describe the neutrophil histologically

Back 38

nucleus is 3-5 lobed and cytoplasm is transparent

Front 39

name function of neutrophil

Back 39

attack and kill bacteria. they are the antibacterial agents.

Front 40

What is the size and percentage of WBC count of eosinophils

Back 40

12 microns and 4% of WBC

Front 41

describe the eosinophils histologically

Back 41

nucleus is bilobed and LM: is pink cytoplasm. TEM: shows cats eye crystals.

Front 42

explain eosinophils major basic protien

Back 42

creates holes in parasitic worms and destroys them

Front 43

explain eosinophils 2 functions

Back 43

reduce inflammatory response
phagocytosis of antigen antibody complex-clean up

Front 44

basophil size and WBC percentage

Back 44

12 microns and less than 1%

Front 45

describe basophil histologically

Back 45

cant see the nucleus very well, it is an S shape. LM: coarse and dark cytoplasm. TEM: large dense secretory vesicles.

Front 46

basophil function and origin

Back 46

inflammatory responces. it is like a mobile mast cell. regulated secretion. origin in bone marrow

Front 47

monocytes size and WBC percentage

Back 47

3-8% largest of the WBC

Front 48

describe the monocytes histologically

Back 48

eccentril oval or kidney bean shaped.

Front 49

monocytes function

Back 49

gives rise to macrophages
forms multinucleated giant cells

Front 50

lymphocytes percentage in WBC

Back 50

20-25% 2nd most common WBC

Front 51

describe lymphocytes histologically

Back 51

nucleus is large and round. LM: thin rim, light blue cytoplam.

Front 52

lymphocytes function

Back 52

immunity, host defense. only WBC to return to blood stream.