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48 Cards in this Set

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What are the two models explaining the hematopoietic stem cell ontogeny in the embro and fetus?
1. Single stem cell model
-- hematopoietic stem cells arise from the yolk sac
-- migrate to the fetal liver and later to BM

2. Dual stem cell model
-- hematopoietic stem cells arise during the mesoderm formation
-- migrate both to the yolk sac and to intraembryonic sites (aorta, gonad, mesonephros)
-- capable of self-renewal and diff in absence of growth factors as opposed to progenitor cells found in adult marrow
-- shown to give rise to several mature cell types (erythrocytes, megakaryocytes, granulocytes, monocytes, anc cells of immune system)
-- present in cord at birth and been shown to engraft and sustain long-term hematopoiesis in transplanted individuals
What are the periods that divide hematopoiesis from coception to birth?


Describe the process of hematopoiesis.
Foci of primitive erythropoiesis are identified by the 3rd to 4th week in the area vasculosa of the yolk sac
-- give rise to the blood vessels and to the primitive blood cells, hematocytoblasts

First blood cells produced by the embryo are RBC series and can be seen 4-5wks post-conception. Characterized by:
1. intravasc differentiation and embryonic Hb content
2. persistence of the nucleus as they mature
3. increased sensitivity to erythropoietin: more rapid differentiation and shortened lifespan
4. megaloblastic appearance w/ MCV of 250fl/cell

Normoblastic erythropoiesis begins around 6th week of gestation in the liver and by 10th week development accounts for >90% of circulating RBCs
-- liver remains major site of hematopoiesis from 3rd to 6th month

By second trimester, gradually shifts from liver to BM
What about hematopoiesis following birth?
Amount of marrow tissue continues to grow w/o increase in cellular concentration.

To increase production:
-- increase cell turnover OR
-- increase volume of hematopoietic tissue producing marrow expansion mostly seen in the calveria
Erythropoiesis is primarily under the influence of...?
The fetus; only partially affected by maternal factors

Erythropoietin, produced by fetal liver plays increasing role in
-- hepatic and myeloid phase
-- will be detectible in cord blood at birth
Erythropoietin levels after birth?
Becomes almost undetectible w/in the first week of life
-- Hb synth and RBC production decline abruptly in first week of life
Hb content in newborns?
Content changes composition throughout embryonic/fetal development and continues through the first few months of life

ε chain --> embryonic Hb
-- declines in first trimester (by second month)

γ-chain --> fetal Hb
-- begins in first trimester
-- peak levels 2nd and 3rd trimesters
-- levels decline after birth for "Hb switch"

β chain --> adult Hb
-- low levels throughout gestation
-- levels increase after birth for "Hb switch"

(See pg 144 for chart)
Normal blood values for a fetus at gestational age?
↑ Hb and Hct during pregnancy w/ stabilization at ~33wks

↓ MCV and ↓ retic count throughout pregnancy
Normal blood values at time of cord clamping?
Placental vessels:
-- 75-125mL of blood (25-33% of fetal blood volume)
-- delayed clamping (30-40sec after birth) ↑ both blood volume (up to 60%) and Hb level
Normal blood values at a sampling site?
At birth, the cap/venous ratio is >1.0 and inversely correlates w/ gestational age
-- >1.2 in premature infants <30 wks gestation

This difference disappears by 5 days life
List causes of anemia in neonates.
Impaired RBC production
Causes of hemorrhage in a neonate?
1. feto-maternal or fetal-fetal occult hemorrhage

2. obstetrical complications/malformation of placenta and cord

3. internal hemorrhage

4. iatrogenic (blood draws)
Causes of feto-maternal hemorrhage?
Most common:
-- traumatic amniocentesis
-- external cephalic version prior to delivery

Other causes:
-- traumatic injury to mother during pregnancy
-- third trimester amniocentesis
-- placental abnormalities
-- manual removal of placenta
Incidence of feto-maternal hemorrhage?
documented in 3/1000 women

Fetal cells will be present in ~50% of all pregnancies
-- when present, account for less than 2mL in 98% of those pregnancies

Hemorrhage ≥ 30mL have been documented in ~3/1000 women
Dx of feto-maternal hemorrhage?
Depends upon the demonstration of fetal cells in the maternal circulation.

In the presence of HbF producing hemoglobinopathies in the mother and ABO incompatibility:

Kleinhauer and Betke acid elucidation technique is most commonly used test
-- based on the resistance of the fetal Hb to elute from intact red cells in acid medium
Incidence of fetal-fetal hemorrhage?
Observed only in monozygotic multiple births w/ monochorial placentas

13-33% twin-twin transfusion in twin pregnancies
-- difference in Hb btwn recipient and donor can be as high as 5g/dL
Result of fetal-fetal hemorrhage?
DONOR: Severe anemia

RECIPIENT: Polycythemia w/ symptoms of hyperviscosity, DIC, and hyperbilirubinemia
Causes of obstetrical complications/malformations of placenta and cord?
Rupture of cord can occur:
-- during rapid delivery
-- in normal delivery when it is too short
-- if entangled around fetus
-- if forceps are used in difficult deliveries

Velamentous insertion of umbilical cord
-- rare
-- 1-2% of pregnancies assoc w/ this malformation result in severe hemorrhage and perinatal death rate ranging from 58% - 80%

Other causes:
-- accidential incision of placenta during C-section
-- placenta previa or abrupto
What can cause internal hemorrhage?
traumatic delivery
vacuum extraction
breech delivery
VitK deficiency
neonatal infection (CMV, adenovirus)
BW <1500g
maternal intake of meds interfering w/ normal hemostasis
maternal platelet Ab directed against fetus' platelets

**anemia occurs in first 24 to 72 hours of life w/o assoc jaundice
Iatrogenic blood draws and anemia?
Number of draws is inversely proportional to birth weight and gestational age
-- more premature will be sickest and require greatest magnitude of interventions

Neonates <1500g lose btwn 5 and 45% of their blood volume during the first 4 weeks in the hospital

Need increased number of transfusions
Clinical manifestations of acute blood loss in a newborn?
acute distress
shallow, rapid, irregular respiration
weak or absent pulses
NO hepatosplenomegaly
Venous pressure of acute blood loss in a newborn?
Lab values of acute blood loss in a newborn?
-- may be normal initially
-- drops rapidly during first 24h of life

normochromic, macrocytic

-- normal at birth

-- rapid treatment of anemia and shock to prevent death

-- IV fluids and blood
-- iron therapy later
Clinical manifestations of chronic blood loss in a newborn?
-- marked pallor disproportionate to evidence of distress
-- occasional CHF, including hepatosplenomegaly
Venous pressure of chronic blood loss in a newborn?
Normal or elevated
Lab values of chronic blood loss in a newborn?
-- low at birth

-- hypochromic, microcytic
-- anisocytosis
-- poikilocytosis

-- low at birth

-- generally uneventful

-- iron therapy
-- PRBCs occasionally necessary
How does hemolysis manifest?
1. persistent increase in reticulocytes w/ or w/o anemia in the absence of hemorrhage

2. rapidly declining Hb w/o increase of reticulocytes and in the absence of hemorrhage
Neonates and bilirubin levels?
For same degree of hemolysis, normal neonates will have higher rates of indirect hyperbilirubinemia (lipid soluble) than an adult counterpart

Seen in 60% of tern infants, 80% premature infants

Normal newborns exhibit a mild elevation of bilirubin in the cord blood w/ a:
-- gradual rise to ~8mg/dl by day 3 to 5 (5-7 in premies)
-- normalization in the second week of life (second month in premies)
Contributing factors to higher bilirubin levels in neonates?
1. increased production or destrucion of RBCs
-- fetal RBC has shorter life than adult RBCs

2. Physiologic hepatic immaturity in performing glucoronidation
-- transferase level at birth is ~1% of adult level and reaches 5% by 3 to 4 days old

3. Overactive enterohepatic recirculation
Possible implications of increased bilirubin levels?
When bili production increases perinatally:
-- rapid rise of indirect bilirubinemia --> most often in 1st 24h of life
-- increased risk of developing kernicterus if not managed rapidly and aggressively
Describe mild hemolytic disease in a newborn/fetus.
Indirect bili: <16-20mg/dL

No anemia

No treatment required

Incidence: 45-60%
Describe moderate hemolytic disease in a newborn/fetus.
No fetal hydrops

Moderate anemia

Severe jaundice -- risk of kernicterus

Treat w/ exchange transfusion

Incidence: 25-30%
Describe severe hemolytic disease in a newborn/fetus.
IN UTERO fetal hydrops
-- incidence of 20-25%

Before 34 weeks 10-12%
After 34 weeks 10-12%

Treat w/ intrauterine transfusion
Name two hemolytic diseases.
1. Rh Hemolytic disease

2. ABO Hemolytic disease
Prevalence of Rh hemolytic disease?
Prevalence w/in 6mos of delivery of the first Rh+ ABO compatible infant is 16%
Immune response of Rh hemolytic disease?
-- slow, 8-9wks
-- weak
-- produces IgM

-- rapid
-- intense
-- produces IgG --> crosses placenta
Pathogenesis of Rh hemolytic disease?
Maternal anti-D IgG Ab (IgG1 and/or IgG3) coats fetal RBCs --> destruction

Persistent extramedullary erythropoiesis
-- spleen, liver
-- kidney, adrenal glands
-- hepatosplenomegaly --> hepatic and cardiac failure
Tx of Rh hemolytic disease for the mother?
1. Prevention of Rh immunization w/ anti-D IgG
-- 300μg after full-term delivery
-- ≥100μg after 2nd trimester abortion
-- 50μg after 1st trimester abortion

2. Suppression of alloimmunization
-- intensive plasma exchange
-- high dose IGIV
Tx of Rh hemolytic disease for the fetus and infant?
1. Intrauterine transfusion
-- blood <96 hrs old irradiated to produce GVHD
-- 40-50mL/kg admin in umbilical vein under ultrasound guidance

2. Exchange transfusion (prevents further hemolysis)
-- Group O or ABO compatible blood used
-- 1.5 - 2 infant blood volues are exchanged (130-170 mL/kg)
-- 90% of RBCs are removed after 2 volumes and 70% after 1 volume
-- not as good in removing bilirubin and IgG

3. phototherapy
Prevalence of ABO hemolytic disease?
--Milder thatn Rh hemolytic disase and ABO incompatibility in adults

-- hydrops fetalis is extremely rare
Pathogenesis of ABO hemolytic disease?
-- fewer A and B Ag on fetal RBCs

-- Anti-A and Anti-B do not bind complement on the fetal RBC mbrn

-- Most Anti-A and Anti-B are IgM and do not cross the placenta

-- IgG Anti-A and Anti-B cross the placenta binding to several other tissues and secretions
Labs of ABO hemolytic disease?
25-30% of ABO-incompatible babies will have a weakly positive DAT on cord blood at delivery
-- often negative on capillary blood 2-3 days later
Tx of ABO hemolytic disease?
Less that 2% of ABO incompatible babies will have disease severe enough to require exchange transfusion
Do any other antibodies cayse fetal hemolytic disease?
Striking reduction in Rh hemolytic disease has been accompanied by an increase in non-D alloimmunization

Ab associated w/ hydrops fetalis are:
-- Anti-Kell
-- Anti-c
-- Anti-Fya
What are the causes of impaired RBC production in a fetus/neonate?
1. physiologic anemia of prematurity

2. Diamond-Blackfan syndrome

3. Others:
-- Pearson's syndrome, Leigh disease, Kearns-Sayre syndrome
-- Congenital infections --> CMV, rubella, adenovirus, parvovirus
-- Congenital leukemia, Down's syndrome, etc.
Describe physiologic anemia of prematurity.
1. ↓↓ RBC mass > expanding plasma volume

2. Combination of physiologic shutdown of hematopoietic activity soon after birth and accelerated destruction of immature RBCs
-- Hb as low as 11.4g/dL in full-term infang at 8-12wks (normal)
-- Hb of 8.0g/dL at 4-8 wks for premature infants <1500g
Treatment for physiologic anemia of prematurity?
Erythropoietin (r-HuEPO) is as effective as allogeneic blood transfusion in increasing Hb level, but much more costly
Describe Diamond-Blackfan Syndrome.
25% of affected patients are anemic at birth
-- Hb <10g/dL
-- reticulocytopenia

Physical anomalies in 30%
-- short stature is most common
-- others: microcephaly, cleft palate, triphalangeal thumb, eye anomalies

Inheritance is uncertain