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10 Cards in this Set
- Front
- Back
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Luminescence |
Decay due to emission |
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Fluorescence is |
Very sensitive |
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Ability to fluorescence depends on |
Aromatics Fused hetero cyclic rings Rigid molecules Substituents |
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What is quantum yield |
Photons out Vs photons in |
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What is fluorescence lifetime |
Average time in excited state |
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What is quenching |
Decrease of fluorescence output |
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What is kash's rule |
Cannot obtain emission that is higher wavelength than source |
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Rayleigh Raman scattering is |
Rayleigh is re emission of photon absorbed by solvent at same wavelength or multiple Raman is remission of photon absorbed by solvent at a longer wavelength due to transfer of vibrational energy |
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Fluorescence advantagea |
Measured directly but for abs it is measured as ratio Lod is blanked limited but for absorbance it is Detector limited |
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Diagram of fluorescence |
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