Mmg Lab M

Front 1

Describe Anabaena

Back 1

-cocci in chains
-gram stains both seen
-4 microns
-found in colonies

Front 2

Describe Bacillus anthracis

Back 2

-bacilli in chains
-Gram +
-from environment and animals
+endospore forming, toxin producing rod, causative agent of anthrax, agent of bioterrorism

Front 3

Describe Bacillus cereus

Back 3

-bacilli in chains
-gram +
-from environment
+has bright yellow, water-soluble pigment, is an endospore former, ubiquitous and common lab plate contaminant, causes gastroenteritis, food poisoning with warmed over food such as rice, toxin producer

Front 4

Describe Bacillus subtilis

Back 4

-bacilli
-Gram +
-aerobe
-forms large, beta-hemolytic clonies on BAP, is an endospore former, is ubiquitous and common lab plate contaminant, is opportunistic pathogen

Front 5

Describe Lactobacillus sp.

Back 5

-bacilli
-Gram +
-from human, animal, and environment
-normal oral, intestinal, and vaginal flora; helps keep foreign microbes in check

Front 6

Describe Enterococcus faecalis.

Back 6

-cocci in chains
-Gram +
-from animal and human
-catalase negative
-alpha hemolytic
-no growth on MAC plate
-white growth on TSA
-formorly thought of as a streptococcal microbe
-normal fecal flora, opportunistic as well as a nosocomial pathogen, associated with VRE problems, used as indicator of animal fecal contamination in water

Front 7

Describe Enterococcus faecium.

Back 7

-cocci in chains
-Gram +
-from animals and humans
-catalase negative
-formorly thought of as Streptococcal microbe
-normal fecal flora, opportunistic as well as a nosocomial pathogen, associated with VRE problmes, used as indicator of animal fecal contamination in water

Front 8

What does VRE stand for?

Back 8

-Vancomycin Resistant Enterococci

Front 9

Describe Escheriachia coli.

Back 9

-Bacilli
-Gram -
-Facultative anaerobe
-from human and animals
-no pigments
-gamma hemolysis
-Lactose + (magenta colony, opaque pink medium) on MAC
-fermenter for O/F Leifson
-grown throughout thio.
-normal fecal flora
-most common cause of community acquired UTI infections in females

Front 10

Describe the E coli strain O157:H7.

Back 10

-Bacilli also
-Gram - also
-special strain of E. coli with additional genetic material which codes for toxins; has been involved in serious outbreaks of feed poisoning, usually associated with beef

Front 11

Describe Micrococcus luteus

Back 11

-cocci in tetrads
-Gram +
-1.0 micrometer
-microaerophile
-polar Orange-Dry water-insoluble pigment
-Gamma hemolysis
-no growth on MAC plate
-inert for O/F Purple
-very purple!

Front 12

Describe Pseudomonas aeruginosa

Back 12

-Bacilli, Gram -
-from hum, an, and environ
-has grape-like smell
-produces pyocyanin (blue green water-soluble pigment)
-beta hemolysis
-beige medium and colonies on MAC (lactose not fermented)
-oxidizer for O/F Leifson
-ubiquitous and especially associated with water
-serious opportunistic and nosocomial pathogen
-associated with wounds (especially burns) and pneumonia

Front 13

Describe Serratia marcescens.

Back 13

-Bacilli
-Gram -
-from human, animal, and environmet
-produces bright red water-insoluble pigment; ubiquitous and found as opportunistic pathogen

Front 14

Describe Staphylococcus aureus.

Back 14

-cocci in clusters (but found as individuals)
-Gram +, from humans
-buttercup gold water insoluble
-no growth on MAC plate
-fermenter for O/F Purple
-catalase +; AKA: Coagulase Positive Staphylococci
-may be beta hemolytic
-causes impetigo and wound infections, serious nosocomial pathogen, causes food poisoning through toxin production, may be resistant to several antimicrobials

Front 15

Describe Streptococcus pneumoniae

Back 15

-diplococci in chains, lancet shaped
-Gram +
-catalase negative
-alpha hemolytic on BAP
-may be normal throat flora, major cause of community acquired pneumonia where sputum appears rut colored, recently associated with neonatal sepsis

Front 16

Describe Streptococcus pyogenes.

Back 16

-Cocci in chains
-Gram +
-from humans and animals
-AKA Group A streptococci
-catalase negative
-beta hemolytic
-causes pharyngitis, strep thoat, impetigo, rheumatic fever, has been described as the flesh eating bacterium

Front 17

Describe Klebsiella pneumoniae.

Back 17

-bacilli
-Gram -
-from human and environment
-among normal fecal flora
-possesses a very large capsule
-causes pneumonia where the sputum appears like "current-jelly"

Front 18

Describe bacteroides fragilis.

Back 18

-bacilli
-Gram -
-from humans and animals
-normal fecal flora and may be most numerous bacteria found in feces
-opportunistic pathogen
-anaerobe for thio (though we found it to be aerobic)