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### 10 Cards in this Set

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 What are two ways of plating diluted bacterial samples? -transfer 0.1 ml a sample to center of sterile agar plate and use sterile hockey stick to spread -transfer 0.1 ml sample to melted agar tube and mix: pour this onto another solid plate and swirl it How long are plates incubated for dilution tests? -24 hours at appropriate temperature How do you find the CFU's of a plate? -count the colonies and locate the "countable plate" (30-300) How do you find the SDF of a dilution problem? -this is the initial sample or flask (sample dilution factor) -if undiluted it would be 1/1 -if 1ml substance is diulted with 9 ml water, the SDF would be 1/10 How do calculate the individual tube dilution factor (ITDF)? -vol transferred/(vol sample transferred + volume diluent) -example: 0.1 transferred to 9.9ml would be a 1/100 ITDF How do you find the Total Seris Dilution Factor (TSDF) for a countable plate? -multiply ITDF's up to tube used *do NOT use tubes that came AFTER the countable plate since it wasn't part of the dilution! How do you find the Plating Dilution Factor (PDF)? -divide amount transferred by 1 ml *always compare this to 1ml! Why do you always compare the plated amount transferred to 1 ml? -because original concentration is CFU/ml How do you find the Final Dilution Factor? -SDF x TSDF x PDF How do you find CFU/ml in ORIGINAL sample? -CFU of countable plate x 1/FDF OR -CFU/FDF