Mmg Lab F

Front 1

What are three things to look for in media before using?

Back 1

1) contamination
2) dehydration
3) excessive moisture

Front 2

What should you label each plate/test tube with?

Back 2

-section & bench #
-Initials/Date
-microbe and inc temp

Front 3

How would you collect a sample of a broth?

Back 3

-gently shake tube
-take sample off top

Front 4

How do you transfer a sample to a broth?

Back 4

-touch sample to surface of broth
-shake tube gently

Front 5

How do you do a solid slant streak?

Back 5

-start streak at base of slant
-contact agar
-zig zag up surface in one continuous motion

Front 6

How do you make a solid slant for a streak AND stab?

Back 6

-place needle at base of slant
-stab the butt of the tube stopping about 1/4" from bottom
-streak slant by drawing needle up the slant in a zig zag motion

Front 7

How do you perform a deep solid?

Back 7

-stab 1/4" from bottom and pull needle out some path
*do NOT make multiple stabs!

Front 8

How do you perform a solid plate?

Back 8

-transfer to zone 1 for confluent growth
-streak as directed

Front 9

Once a slant/broth/plate is made, what is done with the culture?

Back 9

-incubate at approprate temperature for about 24-48 hrs (depends on micro):
human body= 37 C
room= 22 C
Fridge= 4 C
hot springs >55 C
Arctic <-20 C

Front 10

What are the 5 reasons for streaking?

Back 10

-dilute sample for isolated colonies
-separate out a mixture of microorganisms
-observe contamination of pure culture
-observe colonial morphology
-maintain microbes in a pure culture

Front 11

How do you perform a spread plate technique?

Back 11

-place agar plate on turn table
-place sample in very center of plate
-dip hockey stick in dirty and touch flame: remove immediately!
-repeat with clean alcohol
-use capillary action to move sample along stick
-hold spreader gently on surface and start to spin turn table
-after several rotations, start to move the spreader back and forth over surface of plate while still spinning
-resterilize