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21 Cards in this Set
- Front
- Back
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What does ubiquitous mean?
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-found in all natural environments, including plants, animals, soils, and water
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What is contamination?
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-the presence of microbes in undesirable areas
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What are aseptic techniques?
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-precautionary measures used to avoid contamination of cultures
-protect lab worker and environment as well |
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What is aerosol contamination?
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-spatter in air from microbes (can be caused be hot needle in colony)
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What is dilution/iosolation streaking?
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-developed by Loeffler and Gaffky in Koch's lab
-involves dilution of bacteria by systematically streaking them over agar surface to obtain isolated cells |
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What is a colony?
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piles of cells that grow from a "parent" cell
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What is a pure culture?
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-agar surface grows only one type of bacterium that are genetically the same
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What is confluent growth?
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-growth over entire surface of streaked area
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What does sterile mean?
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-means without viable microbial cells present
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What are two ways microorganisms can be transported?
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-live carriers
-dust particles |
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What are some important functions performed by microorganisms?
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-decomposition
-Nitrogen recylcling -food production -drink production -antibiotic production -assistance in digestion |
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What tools are used to transfer small amounts of growth from one medium to another?
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-an inoculating loop and needle
-bunsen burner |
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How long should you cool a loop or needle beofre transferring a culture?
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-atleast 15 seconds
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What happens if you plunge a hot needle into a colony of bacteria on an agar plate?
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-kill bacteria
-spatter agar and microbes into air resulting in aerosol contamination |
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What can be studied from dilution/isolation streaking?
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-colonial morphology
-pigmentation -hemolysis |
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Why should plates be inverted when incubating them?
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-so condensation won't drip on them and disperse forming colonies
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What are three things that should be checked for in media before use?
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-sterility
-excessive moisture -excessive drying |
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Describe the type of growth, pigmentation, and colonly morphology (if applicable) for Serratia marcescens in a TSB broth.
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-no surface growth, diffuse
-white color |
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Describe the type of growth, pigmentation, and colonly morphology (if applicable) for Serratia marcescens in TSA deep.
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-complete growth on stab
-cloudy and translucent -no solubity observed |
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Describe the type of growth, pigmentation, and colonly morphology (if applicable) for Klebsiella pneumoniae on a TSA slant.
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-filiform growth
-tan color -water insoluble |
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Describe the type of growth, pigmentation, and colonly morphology (if applicable) for Bacillus cereus on a TSA plate.
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-type of growth N/A
-yellow colonies, water soluble -1-2 mm circular colonies with undulate adges and pulvinate height -light could not pass through or be deflected and colonies had viscid consistency |
