Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
12 Cards in this Set
- Front
- Back
|
What are some physical ways to control growth of microbes?
|
-Heat
-Pasteurization -ultra-high-temperature -autoclaving -UV light |
|
|
What are some chemical ways of controling microbial growth?
|
-dyes
-antimicrobial agents (such as antibiotics) |
|
|
What are the settings for autoclaving?
|
-121 C
-15 minutes -15 pounds pressure |
|
|
How does UV light work to sterilize things?
|
-light absorbed by purine and pyrimidine bases of DNA, cuasing thymine dimers
-orgnaism can't replicate and dies |
|
|
What are thymine dimers?
|
-the binding of two adjacent thymine residues
|
|
|
What types of organisms are most susceptible to UV light?
Most resistant? |
-actively dividing cells
-spores and pigmented cells |
|
|
What is difficult about UV light?
|
-Poor penetration ability
|
|
|
What is a common dye used in chemical control of microbes?
|
-Crystal Violet combats thrush
|
|
|
What are the two categories of antimicrobials?
|
-bacteriostatic
-bacteriocidal |
|
|
What do antimicrobials work on in the microbe?
|
-cell wall synthesis
-ribosomal action -nucleic acid synthesis *may act on some other cellular site |
|
|
When treating with antibiotics, what must be considered?
|
-degree of susceptibility
-toxicity to host |
|
|
What are 5 ways to test for microbe sensitivity to antimicrobials?
|
1) classic tube dilution
2) micro-dilution method 3) agar plate dilution 4) Kirby-Bauer disc diffusion methods 5) E-test (new) |
