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12 Cards in this Set

  • Front
  • Back
What are some physical ways to control growth of microbes?
-UV light
What are some chemical ways of controling microbial growth?
-antimicrobial agents (such as antibiotics)
What are the settings for autoclaving?
-121 C
-15 minutes
-15 pounds pressure
How does UV light work to sterilize things?
-light absorbed by purine and pyrimidine bases of DNA, cuasing thymine dimers
-orgnaism can't replicate and dies
What are thymine dimers?
-the binding of two adjacent thymine residues
What types of organisms are most susceptible to UV light?
Most resistant?
-actively dividing cells
-spores and pigmented cells
What is difficult about UV light?
-Poor penetration ability
What is a common dye used in chemical control of microbes?
-Crystal Violet combats thrush
What are the two categories of antimicrobials?
What do antimicrobials work on in the microbe?
-cell wall synthesis
-ribosomal action
-nucleic acid synthesis
*may act on some other cellular site
When treating with antibiotics, what must be considered?
-degree of susceptibility
-toxicity to host
What are 5 ways to test for microbe sensitivity to antimicrobials?
1) classic tube dilution
2) micro-dilution method
3) agar plate dilution
4) Kirby-Bauer disc diffusion methods
5) E-test (new)