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33 Cards in this Set

  • Front
  • Back

Positive ONPG

Yellow coloration to disk

Positive Indole

MIO tube: RED, Spot: Turquiose

Positive Urease

Bright pink

Positive PAD

Dark green color

Positive Citrate

Blue coloration

Positive Decarboxylase

Purple for Alkaline

Positive DNase

Pink around colonies

Positive VP-MR

Red color

Positive Nitrate

Red color

What does K and A refer to in a TSI?

A Acidic= yellow


K Alkaline= red

Organisms that cause H2S production on TSI

Proteus


Salmonella


Citrobacter fruendii


Edwardsiella

Sucrose nge, lactose neg (K/A)

Salmonelaa , Shigella

Organisms that are considered intestinal pathogens

Escherichia coli, shigella, salmonella, yersinia

TSI reactions not produced by enterobacteriaeae

A/K, K/K

EHEC causes...

Watery diarrhea progressing to bloody diarrhea

O157:H7 tests:

SMAC - colorless colonies


MUG - negative


Latex - positive


Confirm with Chromagar

Lactose positve organisms are generally...

Negative PAD and Oxidase

E. Coli characteristics

Lac Pos with bile ppt


Indole +


Citrate - *only organism that does this*

Enterobacter characteristics

Ornithine +


Citrate +

Citrobacter freundii characterisitcs

Ornithine -


Citrate +


H2S +

Citrobacter koseri

Ornithine +


Citrate +


H2S -

Klebsiella characteristics

K. Pneumoniae indole -


K. Oxytoca indole +

Serratia charcaterisitcs

DNase positive (pink)

Proteus characterisitcs

Vulgaris. Indole + ornithine -


Mirabilis. Indole - ornithine +

Morganella

Ornithine +

Lactose - & ONPG -

Morganella, Proteus, Providienca, Salmonella

Providencia characteristics

Urease variable


Ornithine -

Shigella characteristics

HEA: Green colonies due to lactose and sucrose negative


Biochemically inert

Salmonella

HEA: Black colonies due to H2S production

Yersinia characteristic

Urease +


CIN agar

Tribe Proteeae

Proteus, providencia, morganella

Bacillary dsyentery

Shigella dysenteriae

Name salmonella species

Salmonella enterica


Salmonella seritype typhi