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170 Cards in this Set
- Front
- Back
What are the reading criteria fpr BA (4)? (Name types of growth)
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(1)no growth evident (2)Beta hemolysis (3)Alpha Hemolysis (4)Gamma hemoylsis
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If the media is BA and there is no growth, what is the microbe?
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cant tell bc no growth
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If the media is BA and there is clear zone around the colony, what is it?
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Beta Hemolysis
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IF the media is BA, and the culture has green decolorazation from the top, what is it?
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Apha hemolysis
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If the media is BA and the culture has no color change in the medium, what is it?
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Gamma hemolysis
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What is gamma hemolysis?
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is in BA and has no color change
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What is Alpha hemolysis?
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is in BA and has green decolorization at the top
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What is Beta hemolysis?
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is found in BA and has a clear zone around the colony
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What are the reading critreia for MAC? (what organimsms can it be) (2)
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(1)fermenter (2)nonlactose fermenter
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What is a fermenter? (2)
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is found in MAC and is pink and red
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What is nonlactose fermentor?
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is found in MAC and is colorless
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If the media is MAC, and it is colorless, what is it?
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nonlactose fermentor
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If the media is MAC, and its color is Pink or red, what is it?
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fermentor
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SA stands for (1)
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Salt agar
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What are some ph incolulators? (3)
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(1)TALU (2)TSI (3)UB
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(1) is a ph indicator
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tMSA
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tMSA is a (1)
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ph indicator
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when is the ph postive?
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when its is phenol red
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What is the reading criteria for MSA? (3) (name organisms it can be)
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(1)Staphylococcus aereus (2) streptococcus epdidermis (3)Staphloycoccus
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when is a organism stapholycoccus aereus?
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if it is in MSA media and if medium growth turns a lemon yellow
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If the media is MSA and the medium growth turned a lemon yellow, What is it?
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Stapholyoccous aereus
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When is the culture Streptococcus epidermis?
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if it is in MSA media and the growth medium is pink
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If the media is MSA and the growth medium is pink, what is the organism?
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Streptococcus epidermis
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When is the organism stapholyoccus?
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if the media is MSA and there is no growth
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If the media is MSA and there is no growth. what is the organism?
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stapoholyococcus
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what organisms can be found in the medium PA ? (2)
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(1)Pseudomonas (+)
(2)Pseudomans (-) |
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When is a culture pseudomanos (+)?
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if the media is PA and the growth is streaked on a line
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What is a culture psedumonas (-)
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if the media is PA and there is no growth
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If the media is PA and there is no growth. what is the organism?
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pseudomonas (-)
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if the media is PA, and there is growth streaked in a line, what is the media?
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Pseudmonas (+)
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What does CIT stand for?
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Simmon's Citrate agar
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What does SIM stand for?
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Sulfide Indole Motility Medium
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What does SIM stand for?
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Sulfide Indole Motility Medium
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What does TSI stand for?
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triple Sugar Iron
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What is the purpose of CIT?
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dectection of citrate utilizers
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NAME
purpose is the decection of citrate utilizers |
CIT
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What is catergory of CIT?
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biochemical
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What is the catergory of SIM?
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biochemical
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What is the catergory of TSI?
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biochemical
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What is the catergory of UB?
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biochemical
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What medias are under the caterogry biochemical? (4)
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(1)CIT (2)SIM (3)TSI (4)UB
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What is the purpose of SIM?
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screening for indole and H2S production and motility
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NAME
purpose is the sceening for indole and H2S production and motality |
SIM
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What is the purpsoe of TSI?
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screening for fermentative ability
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NAME
purpose is for the screening of fermenative ability |
TSI
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What is the purpose of UB?
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producation of exoenzyme urease
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NAME
purpose is the production of a exoexnzyme urease |
UB
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What does UB stand for?
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Urea Broth
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If the culture is in a UB media, what organisms can be found? (2)
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(1)urease (+) (2)urease (-)
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When is the culture urease (+)?
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if it is in the media UB and is fuschia
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when is the culture urease (+)
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if the media is UB and a fuchsia color
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IF the media is UB and the culture is fuchsia color, what is the organism?
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urease (+)
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if the media is UB, and the culture is a orange color, what is the media?
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urease (-)
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What is the purpose of UV radiation in a experiment? and how is it limited?
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(1)has a lethal effect on bacteria and can therefore be used as a sterilzing agent (2)bc bacteria can repair uv damage
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UV light can cause the (1)
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formation of thymine dimers
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What are enzyme is used in the light repair?
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DNA photoylase
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What enzymes are used in dark repair?
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(1)helicase (2)DNA polymerase (3)DNA Ligase (4) endonuclease
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What is the function of helicase in dark repair?
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removes nucleotides
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What is the function of endonuclease in dark repair?
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detects the thymine dimer
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What is the function of DNA polymerase in dark repair?
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inserts complementray DNA nucleotides
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What is the function of ligase in dark repair?
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seals the sugar phosphate bone
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What is the function of DNA photolyase?
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a repair enzyme that reversese the reaction
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E coli can perform (1)repair
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both light and dark
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What is the kirby and baver test?
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a culture and senstivity test that measures the zone of inhibition
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NAME
is a culture and senstivity test that measures the zone of inhibition |
kirby and baver test
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NAME
is called photoreaction |
light repair
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NAME
is called excision repair |
dark repair
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What is another name for dark repair?
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excision repair
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What does STA stand for?
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Snyder's Test Agar
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What is the purpose of STA?
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dectect and ID lactobacilli
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What is the catergory for STA?
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Selective and differintial
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NAME
the purpose of this media is to dectect and ID lactobacilli |
STA
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What is STA ph indicator?
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bromcresol green
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Bromcresol green in (1) ph indicator
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STA
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What does LSB stand for?
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Lauryl Sulfate broth
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what is the purpose of LSB?
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detect and ID coliforms in food
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NAME
the purpose of this media is to detect and ID coliforms in food |
LSB
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What caterogty is LSB ?
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differential and selective
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How do you tell what microbe it is if it is a LSB media?
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(1)if there is a gas buble in the durham's tube then it is "coliforms +" (2)if there is no gas then it is coliforms - "
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If the microbe is in a LSB media, and has a gas bubble in the Durham's tube, what is it?
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coliforms +
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If the microbe is in a LSB media, and has no gas bubbles, what is it?
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coliforms -
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NAME MEDIA
the microbe was found to be coliform + |
LSB
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What is the bacterial lawn?
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space around the anitbiotics
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What does BEA stand for?
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Bile Escular Agar
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What is BEA's catergory?
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Selective and differential
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What does EMB stand for?
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Eiosan metheylene Blue
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NAME
is a "fecal contmination indicator" |
E coli
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NAME
has a red pigmentation and causes pneumonia in alcholics and in wounds of burn patients |
Serrshatia marcesens
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What is transformation?
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when live bacteria absorbs DNA fragments and expressises new traits
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What is serrshatia marcescens? (2)
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(1)has red pigmentation (2)causes pneumonia in alcholics and in the wounds of burn patients
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What is the purpose for EMB?
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to detect califorms which detect fecal contamination
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If it is in a UB media and is a orange color, What is it?
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urease -
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If it is in UB media and is fuschia color, What is it?
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urease +
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If it is in CIT media and is a Blue color, What is it?
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Citrate +
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If the media is SIM and it is a black color, what is it?
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H2S+
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If the medida is CIT and the media is green, What is it?
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Citrate -
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If the media is SIM, and it is cloudy, What can you conclude?
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moltitly+
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If the media is SIM, and you add (1) and turns red, What is its readings?
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(1)Kovac (2)idole +
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If the media is TSI, and the slant is red,, What is it?
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K for alakaline
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If the media is TSI, the slant is yellow, What are the readings?
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A for acid
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If the media is TSI and the butt is red, what is the readings?
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K
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If the media is TSI and the butt is yellow or black, what are the readings?
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A
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If the media is TSI, the slant is red, the butt is yellow, and there is no gas production, what are the readings?
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(1)AK+ (2)only glucose is fermented
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In TSI, when is there only glucose fermented?
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red=slant and yellow butt
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In TSI, when is there Glucose and lactose fermented and or sucrease feremented?
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yellow slant and butt
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How do you tell if there is no H2S production?
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no black
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What is the Blood order?
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Never Let Monkeys Eat Bannanas
N=neutrophillis L=Lymphocytes M=monocyetes E=Eosoisinophilis B=Basophilis |
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If a person has a fungal infection, what are the max types of blood cells present?
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Esoisinohilis
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If a person has a infection caused by a Helimenth, what are the max types of blood cells present?
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Esuisinophillis
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If a person has increased levels of histermenes resulting in incresased allergies, what are the max types of blood cells present?
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Basohilis
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If a patient has increased levels of Basohilis blood cells, what do they likely have?
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allergies and increased levels of histomenes
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If a patient has increased levels of monocytes, what do they likly have?
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is non-specific nad increase w any infection
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NAME
these blood cells increase when a person has a infection |
monocytes
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If a patient has increased levels of Esosinophilis blood cells, what do they likely have?
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when you have a fungal infection by Hemimeths
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If a patient has increased levels of Neutrophilis in their blood, What do they likely have?
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bacterial infection
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A patient has a bacterial infection, what are the max types of blood cells present?
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Neutrophilis
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If a patient has increased levels of lymphocytes, what do they likely have?
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a viral infection
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A patient has a viral infection, what are the max types of blood cells present?
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lymphocytes
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What is Blood types A? (4)
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(1)has Anigen A (2)the Anitbody in plasma is Anti-B (3)Rxt w Anti A =+(4)Rxt w AntiB= -
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What is Blood type B?(4)
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(1)has antigen B (2)Anitbody in the plasma is anti A (3)Rxt w Anti B = - (4) Rxt w Anti B=+
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What is Blood types AB? (4)
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(1)has antigens A and B (2)is not anti anything (3)Rxt w Anti A = + (4) Rxt w Anti-B=-/+
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What is blood type O?
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(1)has nether A or B antigens (2)Rxt w Anti-A= - (3)Rxt w Anti-B=-
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for the blood types, which ones are domminant and recessive of the O, A, and B?
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A and B are co-dominant and O is recessive
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Which medias are used for unkown projects?
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(1)MAC (2)CIT (3)GLU (4)SIM (5)UB (6)TSI
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Which medias are used for food?
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(1)EMB (2)LSB (3)MSA (4)PA (5)TSA
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Which media is used to test for dental carries?
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STA
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NAME
category is general |
TSA
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NAME
category is Selective and Differiental (4) |
(1)EMB (2)MAC (3)MSA (4)STA
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NAME
category is biochemical (7) |
(1)CIT (2)GLU (3)SIM (4)UB (5)TSI (6)LSB (7)PA
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What are the specail ingredents in MSA?
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salt
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Which media's contain Durham tubes? (2)
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(1)GLU (2)LSB
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Which medias have a low ph?
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STA
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Which medias are pH indicators?
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(1)UB, TSI, and MSA= phenol red (2)Bromcresol green=STA
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What is the pH indicator for STA?
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bromcresol green
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What is the pH indicator for UB, TSI, and MSA?
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phenol red
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NAME
media is a respiration/ fermentation test |
TSI
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TSI is a (1) test
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respiration/fermentation
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NAME
media is used to test milk quailty |
Methylene Blue Reductase test
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What is Methylene Blue Reducatse test used to do?
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test the quaility of milk
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For the Methylene Blue Reductase test, how do you tell if the milk is good or not?
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(1)if it changed from blue to white in under two hours= poor quailty of milk (2) if changed after 6 hours=good quaility
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For the Methylene Blue Reductase Test, blue=? and white=?
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(1)oxidized (2)reduced
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What is the media LSB and ESB used for?
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to find fecal contamination
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What is the media MSA used for?
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handling
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What is the media PA used for?
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spoilage of water
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What is the media TSA used for?
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general bacteria count in food
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NAME MEDIA
is used to find fecal contamination? (2) |
LSB and ESB
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NAME MEDIA
is used to test foods for handling |
MSA
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NAME MEDIA
is used to count baceteria in the food |
TSA
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What media where used to test Iced Tea?
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(1)EMB (2)LSB (3)PA (4)TSA
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EMB, LSB, PA, and TSA used for??
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to test iced tea
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Why was not MSA used to test iced Tea?
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bc it contains tannic acid which would interact w salts and give you a fasle postive
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In the transformation experiment, why did we include ampiciillin in the medium?
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to show the transformed cells
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NAME
is antibiotic senstivity test |
Kirby-Bauer test
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What does aggulination mean?
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clumping
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what increased pathological smear of mono blood ?
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Basisphis
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What reagent is required for the reading of SIM?
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Kovacs
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What are the readings for CIT?
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(1)Blue= citrate +
(2)Green = citrate - |
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What are the readings for SIM? (3)
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(1)black=H2S+ (2)Cloudniss at inculation line=moltitly + (3)when Kalvoc is added and it turns red it is indole +
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What are the readings for GLU?
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(1)Red= K or yellow = A (2) gas = + or -
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What are the readings for MAC?
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(1)Pink/red= fermenator
(2)colorless= nonlactose fermenator |
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What is the purpose of BA?
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test for hemolytic acitivty
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NAME
purpose is to test for hemolytic activity |
BA
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What are the readings for MSA>
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(1)S. aureous =yellow/lemon
(2)pink= s. epidermis (3)no growth = stapholycoys - |
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What is the purpose of MAC?
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isolate gram - enterics
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NAME
purpose to isolate gram - enterics |
MAC
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What is the purpose of MSA?
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isolte Staph species
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NAME
purpose is to isolate staph species |
MSA
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NAME
purpose to isolate gram - enterics |
MAC
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What is the purpose of MSA?
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isolte Staph species
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NAME
purpose is to isolate staph species |
MSA
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