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32 Cards in this Set
- Front
- Back
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Guanine |
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Adenine
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Thymine
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Cytosine
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Uracil |
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Diameter of DNA |
20 Å |
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1 turn in DNA |
34Å |
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Major grove in DNA |
22Å |
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Minor groove in DNA |
12 Å |
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Distance b/w 2 bases in DNA |
3.4 Å |
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Hershey and Chase Blender experiment with bacteriophages |
found out that DNA is the one that carries information for a new generation of a phage radiolabelled P^32 |
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nucleoside |
SUGAR AND BASE |
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nucleotide |
sugar, base, phosphate |
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How do you form 5 methyl cytosine |
methylation of the 5th carbon on cytosine |
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what happens if you deaminate cytosine? |
uracil |
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what happens if you deaminate 5 methyl cytosine? |
thymine |
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Nucleosides can be in syn and anti... Which one is most prevalent in what type of DNA |
Syn in Z DNA |
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nomenclature: Purines |
-osine |
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nomenclature: pyrimidine |
-idine |
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Major vs. Minor groove |
* major: rich in chemical information
* minor: less a.a. side chains |
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B DNA |
* bases in center
* found under physiological conditions |
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A DNA |
* stubbier
* less hydrated * helices: RNA-RNA, DNA-RNA * hole in center |
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Z DNA |
* L handed
* zig zag * only in prokaryotes * purines in SYN |
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stability of dna |
1. H bonds
2. base stacking |
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Denaturation of DNA |
high temp high pH |
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UV absoption ss vs. ds |
260 nm ds absobs less bc of base stacking ss absorbs 40% more |
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Tm |
50% of the DNA has melted |
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What 2 factors increase Tm? |
1. G:C concentration 2. Salt |
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what is the Tm formula? |
Tm=( 4 deg x (G+C)) x (2 deg x (A+T)) |
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High pH: DNA, RNA |
DNA= denaturation RNA= phosphodiester bonds break bc of 2* OH |
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low pH and DNA |
hydrolysis breaks your DNA into bits |
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What is the wobble? |
G:U pairing in tRNAs |
