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54 Cards in this Set

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  • Back
Describe the gram stain for streptococcus
They are gram positive. They are spherical or ovoid in shape and tend to grow in pairs or chains.
Are streptococci motile or nonmotile? spore forming or nonspore forming?
They are nonmotile, nonspore forming
Describe the need for air of streptococci
They are facultatively anaerobic
What are the most important streptococcus pathogens?
Streptococcus pyogenes (group A streptococcus), Streptococcus agalactiae (group B streptococcus), and Streptococcus pneumoniae (pneumococcus)
What is the other name for group A streptococcus?
Streptococcus pyogenes
What is the other name for group B streptococcus?
Streptococcus agalactiae
What is the other name for pneumococcus?
Streptococcus pneumoniae
What group used to be part of streptococci?
Enterococci
What are the sites of colonization of S. pneumoniae?
Oropharynx, nose
What are the sites of colonization of S. pyogenes?
Nares, pharynx, rectum
What are the sites of colonization of Enterococcus faecalis and faecium?
GI tract
What are the sites of colonization of S. agalactiae?
GU tract
What are the sites of colonization of Viridans streptococcus?
Oropharynx
What bacteria species colonize the oropharynx?
S. pneumoniae, Viridans streptococcus
What bacteria species colonize the nose?
S. pneumoniae
What bacteria species colonize the nares?
S. pyogenes
What bacteria species colonize the pharynx?
S. pyogenes
What bacteria species colonize the rectum?
S. pyogenes
What bacteria species colonize the GI tract?
Enterococcus faecalis and faecium
What bacteria species colonize the GU tract?
S. agalactiae
What are the common sites of infection for S. pneumoniae?
lungs, sinuses, middle ear,
meninges
What are the common sites of infection for S. pyogenes?
pharynx, skin, soft tissue
What are the common sites of infection for Enterococcus faecalis and faecium?
urinary tract, biliary tract,
peritoneum, heart valves
What are the common sites of infection for S. agalactiae?
neonatal, bloodstream, lung and
meninges. genitourinary tract
What are the common sites of infection for Viridans streptococcus?
dental caries, heart valves,
bloodstream
What bacteria often infect the lungs?
S. pneumoniae, S. agalactiae
What bacteria often infect the middle ear?
S. pneumoniae
What bacteria often infect the meninges?
S. pneumoniae, S. agalactiae
What bacteria often infect the pharynx?
S. pyogenes
What bacteria often infect the skin?
S. pyogenes
What bacteria often infect soft tissue?
S. pyogenes
What bacteria often infect the GU tract?
Enterococcus faecalis
and faecium, S. agalactiae
What bacteria often infect the biliary tract?
Enterococcus faecalis
and faecium
What bacteria often infect the peritoneum tract?
Enterococcus faecalis
and faecium
What bacteria often infect the heart valves?
Enterococcus faecalis
and faecium, Viridans streptococcus
What bacteria are often responsible for neonatal infections?
S. agalactiae
What bacteria infect the bloodstream?
S. agalactiae, Viridans streptococcus
What bacteria are associated with dental caries?
Viridans streptococcus
How are streptococci classified?
Through hemolytic pattern or Lancefield grouping
What is hemolytic typing?
Hemolytic typing has to do with the type of hemolysing that occurs

alpha - green hemolysis
beta - clear hemolysis
gamma - no hemolysis
What is Lancefield grouping?
It is based on the cell wall carbohydrate antigens
Classify S. pneumonia
Optochin disk: S
Bacitracin disk: R
Growth in 6.5% NaCl: -
Hydrolyze Bile-Esculin: -
Hippurate hydrolysis: -
Classify S. pyogenes
Optochin disk: R
Bacitracin disk: S
Growth in 6.5% NaCl: -
Hydrolyze Bile-Esculin: -
Hippurate hydrolysis: -
Classify E. faecalis, faecium
Optochin disk: R
Bacitracin disk: R
Growth in 6.5% NaCl: +
Hydrolyze Bile-Esculin: +
Hippurate hydrolysis: -
Classify S. agalactiae
Optochin disk: R
Bacitracin disk: R
Growth in 6.5% NaCl: -
Hydrolyze Bile-Esculin: -
Hippurate hydrolysis: +
Classify Viridans streptococcus
Optochin disk: R
Bacitracin disk: R
Growth in 6.5% NaCl: -
Hydrolyze Bile-Esculin: -
Hippurate hydrolysis: -
What is the outer surface of group A streptococcus made from?
A hyaluronic acid capsule that interferes with phagocytosis
What is the M protein? What does it do?
The M protein is the major virulence factor of group A streptococcus.

The protein interferes with phagocytosis and strains defective in M protein are avirulent. The M proteins appear to promote colonization of tissue surfaces. M proteins are also used to classify GAS based on their variation in structure and sequence. The structure is filamentous and is an alpha-helical coiled-coiled structure. Immunity to infection with GAS is type-specific and is based on the antiphagocytic moiety of the M protein. The antigenically variable regions of the protein are found at the amino terminus and are the most distal. M protein has also been implicated in the pathogenesis of rheumatic fever.
What does protein F1 do?
Together with lipoteichoic acid mediates binding to fibronectin, a host extracellular matrix molecule found on the surface of epithelial cells.
What does lipoteichoic acid do?
Together with Protein F1 mediates binding to fibronectin, a host extracellular matrix molecule found on the surface of epithelial cells.
What does Protein G do?
It binds to the Fc portion of immunoglobulin (similar to protein A in S. aureus)
What findings are suggestive of GpA strep? What are not suggestive?
Suggestive: Sore throat, sudden onset, fever, pain with swallowing, headache, lymphadenitis, tonsillar exudates, soft palate petechiae

Not suggestive: Conjunctivitis, coryza, cough, diarrhea
Whats the difference between S. aureas and Strep TSS?
Strep differs from S. aureus TSS because of the frequent presence of infection.
What is the pathogenesis of the pneumococcal pneumonia from S. pneumo
-Adheres to type II cells and initiates immune response
-Cell wall causes inflammatory response
-Fluid accumulation-> Congestion
-Red hepatization (leakage of RBCs)
-Gray hepatization (recruitment of macrophages)
-Resolution of pneumonia starts with development of anticapsular antibody