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40 Cards in this Set
- Front
- Back
List 4 physical agents for controlling microbial growth.
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1. UV radiation
2. boiling water 3. HEPA filters 4. H2O2 |
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Bacterial endospores have the highest resistance to physical and chemical controls. TRUE/FALSE
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TRUE
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The process that destroys or removes all microorganisms and microbial forms including bacterial endospores is...
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sterilization
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The process of using a cleansing technique to mechanically remove and reduce microorganisms and debris to safe levels is...
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degermation
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The use of a physical or chemical process to destroy vegetative pathogens is...
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disinfection
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The use of chemical agents directly on exposed body surfaces to destroy or inhibit vegetative pathogens is...
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antisepsis
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Sporocides acn be used to acheive sterility. TRUE/FALSE
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TRUE.
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Regarding the rate of microbial death, one can say that cells in a culture die at a constant rate. TRUE/FALSE
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TRUE.
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Sterilization is acheived by...
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steam autoclave
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Dry heat is less effecient...
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than moist heat.
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List 4 ways of killing endospores.
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1. Dry heat at 170C for 2 hours
2. incineration 3. glutaraldehyde for 3 or more hours 4. ethylene oxide for 3 or more hours |
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What are the minimal sterilizing conditions in a steam autoclave?
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121C at 15psi for 15min.
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The shortest time required to kill all the microbes in a sample at a specified temperature is called the...
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thermal death time(TDT)
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Disinfection of beverages such as apple juice, milk and wine is optimally acheived by...
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pasteurization
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Electrons are ejected from atoms in cells when organisms are exposed to...
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gamma and X rays.
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List 4 heavy metals used for antisepsis.
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1. tincture of iodine
2. Merthiolate 3. silver nitrate solutions 4. mercurochrome |
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vegetative bacteria and fungi are the easiest microbial forms to kill or inhibit are...
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vegetative bacteria and fungi.
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List 4 compounds that are phenols.
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1. Lysol
2. hexachlorophene 3. cresols 4. chloramines |
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List 4 factors of hypochlorites.
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1. release hypochlorous acid in solution
2. cause denaturation of enzymes 3. used to disinfect dairy, restaurant and medical equipment 4. found in bleach |
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List 4 substances used as an antiseptic.
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1. iodophor
2. chlorhexadine 3. 3% H2O2 4. merthiolate |
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Betadine is considered an iodophor. TRUE/FALSE
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TRUE
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The compound that is an organic base containing chlorine and 2 phenolic rings, and is used increasingly for wound degerming is...
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chlorhexadine
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List 4 characteristics of alcohol.
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1. denature proteins when in a 50-95% alcohol water solution
2. disinfect items soaked in alcohol 3. are skin degerming agents 4. at 50% or higher concentrations dissolve cell membrane lipids |
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List 4 alkylating control agents.
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ethylene oxide
glutaraldehyde formaldehyde propylene oxide |
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The sterilizing gas used in chemiclaves is...
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ethylene oxide
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The chemical agent that produces highly reactive hydroxyl free radicals and also decomposes to O2 gas is...
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H2O2
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List 4 agents that act as surfactants.
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detergents
soaps quartenary ammonia |
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List several cellular targets of antimicrobials.
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cell wall
cell membrane ribosomes cellular proteins |
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Placing organisms at 4C is...
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bacterioststic
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Pasteurization reduces the number of vegetative forms. TRUE/FALSE
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TRUE
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Gamma, X rays and cathode are forms of radiation in order from the most penetrating to the least penetrating. TRUE/FALSE
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TRUE
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Filtration sterilization removes viruses. TRUE/FALSE
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TRUE
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Vials of microorganisms that undergo the freeze drying process called________ will remain preserved and viable for years.
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lyophilization
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A method for sterilizing milk, called_______ treatment, uses 134C for 1-2 sec.
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ultra high temperature
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Ultraviolet radiation excites atoms to a higher energy state within molecules such as DNA that then leads to the formation of pyrimidine dimers. TRUE/FALSE
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TRUE
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Chlorine is a halogen used in gaseous and liquid form for large scale disinfection of drinking water and sewage. TRUE/FALSE
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TRUE
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______ solution was introduced in the late 19th century for preventing gonococcal infections in a newborns eye after exposure to the mothers infected birth canal.
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AgNO3
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Saliva and pus can interfere with the actions of disinfectants. TRUE/FALSE
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TRUE
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Sonication dislodges debris and saliva from instruments but will not reliably acheive disinfection or sterilization. TRUE/FALSE
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TRUE
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Aniline dyes like crystal violet have antimicrobial activity particularly against G+ bacteria and some fungi. TRUE/FALSE
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TRUE
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