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55 Cards in this Set

  • Front
  • Back
What are the 5 I's of studying microorganisms?
1.inoculation
2.incubation
3.isolation
4.inspection
5.identification
List 4 examples of different types of microbiological media.
broth
enriched
agar
gel
What is the term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium?
inoculation.
Which is essential for development of discrete, isolated colonies?
solid medium
A pure culture contains only...
one species of microorganism
What type of medium will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?
a solid medium
Agar is an important component of media because...
agar provides a solid surface for bacterial growth
What is a contaminated culture?
When one organism growing in a medium is somehow contaminated with another.
Bacterioides is inoculated into a pour plate. After incubation, colonies are only seen within the agar and not on the surface. Bacterioides must be...
anaerobic.
A microbiologist inoculates Staph. epidermidis and E. coli into a culture medium. Following incubation, only the E. coli grows in the culture. what is the explanation?
The culture medium must be selective.
Which method often results in colonies developing down throughout the agar and some colonies on the surface?
pour plate.
A common medium used for growing fastidious bacteria is...
Blood agar.
A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible, would be termed...
synthetic.
A reducing medium contains...
substances that remove oxygen.
Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?
differential
When one uses a nutrient medium that contains thioglycollic acid, what type of microbe is one attempting to culture?
anaerobe
List 4 growth characteristics that occur using differential media?
1.different color colonies
2.different media color post incubation
3.precipitates
4.gas bubbles
A reducing media is used to culture...
anaerobic organisms
For which bacterial genus is mannitol salt agar selective?
Staphylococcus.
In culturing a patients feces for intestinal pathogens, what is likely to be present in selective media for analyzing this fecal specimen?
bile salts
What is a typical characteristic that refers to the microscopes ability to show two separate entities as separate and distinct?
resolving power
What part of the microscope magnifies the specimen to produce the real image of the specimen?
objective lens
What magnifies the specimen to produce the virtual image of the specimen?
ocular lens
If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?
95X
What is the limit of cell diameter that would be resolved in a microscope/
0.2um
The wavelength of light used plus the numerical aperture governs...
resolution
The type of microscope in which you would see brightly illuminated specimens against a black background is...
dark-field
This microscope does not employ light in forming the specimen image:
electron. It also achieves the greatest resolution and highest magnification.
This scope shows cells agaist a bright background and also shows intracellular structures of unstained cells based on their varying densities:
phase-contrast
This microscope cannot image live specimens:
scanning electron
This scope is the most widely used and shows cells against a bright background:
bright-field
List 4 characteristics that pertain to the flourescence microscope?
1.type of compound microscope
2.requires the use of dyes like acridine and fluorescein
3.commonly used to diagnose certain infections
4.requires an ultraviolet radiation source
Which microscope bombards a whole metal coated specimen with electrons moving back and forth over it?
scanning electron
The specimen preparation that that is best for viewing cell motility is..
hanging drop
The Gram stain, acid fast stain and endospore stain have the following in common:
outcome based on cell wall differences
Basic dyes are...
attracted to the acidic substances of bacterial cells
A biologist makes a fixed smear of bacterial cells and stains them with Loefflers methylene blue. All the cells appear blue under the oil lens. This is an example of:
simple staining
The 3 physical forms of laboratory media are:
solid/semisolid/liquid
Bacteria that require special growth factors and complex nutrients are termed...
fastidious
This is a term used for a culture made from one isolated colony...
both axenic and pure
Newly inoculated cultures must be_______ at a specific temperture and time to encourage growth.
incubated
The _____ of the microscope holds and allows selection of the objective lenses.
stage
______ dyes have positive charges on the chromophore and are repelled by bacterial cells.
acidic
______ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.
immersion oil
The procedures for culturing a microorganism requires the use of a microscope. TRUE/FALSE
FALSE
One colony typically develops from the growth of several parent bacterial cells. TRUE/FALSE
FALSE
Some microbes are not capable of growing on artificial media. TRUE/FALSE
TRUE
Mixed cultures are also contyaminated cultures. TRUE/FALSE
FALSE
A medium that is gel-like has less agar in it compared to a solid medium. TRUE/FALSE
TRUE
A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes. TRUE/FALSE
TRUE
A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe. TRUE/FALSE
FALSE
Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens. TRUE/FALSE
TRUE
The bending of light rays as they pass from one medium to another is called refraction. TRUE/FALSE
TRUE
At the end of the Gram stain, Gram positive bacteria will be seen as pink cells. TRUE/FALSE
FALSE
Scanning tunneling microscopes can give close up views of DNA molecules, while the scanning probe microscopes have the potential to see individual atoms. TRUE/FALSE
FALSE