Micro/talaro

Front 1

What are the 5 I's of studying microorganisms?

Back 1

1.inoculation
2.incubation
3.isolation
4.inspection
5.identification

Front 2

List 4 examples of different types of microbiological media.

Back 2

broth
enriched
agar
gel

Front 3

What is the term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium?

Back 3

inoculation.

Front 4

Which is essential for development of discrete, isolated colonies?

Back 4

solid medium

Front 5

A pure culture contains only...

Back 5

one species of microorganism

Front 6

What type of medium will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?

Back 6

a solid medium

Front 7

Agar is an important component of media because...

Back 7

agar provides a solid surface for bacterial growth

Front 8

What is a contaminated culture?

Back 8

When one organism growing in a medium is somehow contaminated with another.

Front 9

Bacterioides is inoculated into a pour plate. After incubation, colonies are only seen within the agar and not on the surface. Bacterioides must be...

Back 9

anaerobic.

Front 10

A microbiologist inoculates Staph. epidermidis and E. coli into a culture medium. Following incubation, only the E. coli grows in the culture. what is the explanation?

Back 10

The culture medium must be selective.

Front 11

Which method often results in colonies developing down throughout the agar and some colonies on the surface?

Back 11

pour plate.

Front 12

A common medium used for growing fastidious bacteria is...

Back 12

Blood agar.

Front 13

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible, would be termed...

Back 13

synthetic.

Front 14

A reducing medium contains...

Back 14

substances that remove oxygen.

Front 15

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Back 15

differential

Front 16

When one uses a nutrient medium that contains thioglycollic acid, what type of microbe is one attempting to culture?

Back 16

anaerobe

Front 17

List 4 growth characteristics that occur using differential media?

Back 17

1.different color colonies
2.different media color post incubation
3.precipitates
4.gas bubbles

Front 18

A reducing media is used to culture...

Back 18

anaerobic organisms

Front 19

For which bacterial genus is mannitol salt agar selective?

Back 19

Staphylococcus.

Front 20

In culturing a patients feces for intestinal pathogens, what is likely to be present in selective media for analyzing this fecal specimen?

Back 20

bile salts

Front 21

What is a typical characteristic that refers to the microscopes ability to show two separate entities as separate and distinct?

Back 21

resolving power

Front 22

What part of the microscope magnifies the specimen to produce the real image of the specimen?

Back 22

objective lens

Front 23

What magnifies the specimen to produce the virtual image of the specimen?

Back 23

ocular lens

Front 24

If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?

Back 24

95X

Front 25

What is the limit of cell diameter that would be resolved in a microscope/

Back 25

0.2um

Front 26

The wavelength of light used plus the numerical aperture governs...

Back 26

resolution

Front 27

The type of microscope in which you would see brightly illuminated specimens against a black background is...

Back 27

dark-field

Front 28

This microscope does not employ light in forming the specimen image:

Back 28

electron. It also achieves the greatest resolution and highest magnification.

Front 29

This scope shows cells agaist a bright background and also shows intracellular structures of unstained cells based on their varying densities:

Back 29

phase-contrast

Front 30

This microscope cannot image live specimens:

Back 30

scanning electron

Front 31

This scope is the most widely used and shows cells against a bright background:

Back 31

bright-field

Front 32

List 4 characteristics that pertain to the flourescence microscope?

Back 32

1.type of compound microscope
2.requires the use of dyes like acridine and fluorescein
3.commonly used to diagnose certain infections
4.requires an ultraviolet radiation source

Front 33

Which microscope bombards a whole metal coated specimen with electrons moving back and forth over it?

Back 33

scanning electron

Front 34

The specimen preparation that that is best for viewing cell motility is..

Back 34

hanging drop

Front 35

The Gram stain, acid fast stain and endospore stain have the following in common:

Back 35

outcome based on cell wall differences

Front 36

Basic dyes are...

Back 36

attracted to the acidic substances of bacterial cells

Front 37

A biologist makes a fixed smear of bacterial cells and stains them with Loefflers methylene blue. All the cells appear blue under the oil lens. This is an example of:

Back 37

simple staining

Front 38

The 3 physical forms of laboratory media are:

Back 38

solid/semisolid/liquid

Front 39

Bacteria that require special growth factors and complex nutrients are termed...

Back 39

fastidious

Front 40

This is a term used for a culture made from one isolated colony...

Back 40

both axenic and pure

Front 41

Newly inoculated cultures must be_______ at a specific temperture and time to encourage growth.

Back 41

incubated

Front 42

The _____ of the microscope holds and allows selection of the objective lenses.

Back 42

stage

Front 43

______ dyes have positive charges on the chromophore and are repelled by bacterial cells.

Back 43

acidic

Front 44

______ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

Back 44

immersion oil

Front 45

The procedures for culturing a microorganism requires the use of a microscope. TRUE/FALSE

Back 45

FALSE

Front 46

One colony typically develops from the growth of several parent bacterial cells. TRUE/FALSE

Back 46

FALSE

Front 47

Some microbes are not capable of growing on artificial media. TRUE/FALSE

Back 47

TRUE

Front 48

Mixed cultures are also contyaminated cultures. TRUE/FALSE

Back 48

FALSE

Front 49

A medium that is gel-like has less agar in it compared to a solid medium. TRUE/FALSE

Back 49

TRUE

Front 50

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes. TRUE/FALSE

Back 50

TRUE

Front 51

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe. TRUE/FALSE

Back 51

FALSE

Front 52

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens. TRUE/FALSE

Back 52

TRUE

Front 53

The bending of light rays as they pass from one medium to another is called refraction. TRUE/FALSE

Back 53

TRUE

Front 54

At the end of the Gram stain, Gram positive bacteria will be seen as pink cells. TRUE/FALSE

Back 54

FALSE

Front 55

Scanning tunneling microscopes can give close up views of DNA molecules, while the scanning probe microscopes have the potential to see individual atoms. TRUE/FALSE

Back 55

FALSE