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10 Cards in this Set
- Front
- Back
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Kinyoun Acid Fast Bacteria
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-detects acid-fast mycobacteria
-carbol fuchsin stains the mycobacteria red and the counterstain is methylene blue |
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Ziel-Neelsen Acid Fast Bacteria
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-detects acid-fast mycobacteria
-carbol fuchsin stains the mycobacteria red and the counterstain is methylene blue -different from the Kinyoun b/c of the carbol fuschin solution |
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Fite Acid Fast Bacteria
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-detects leprosy organisms (Mycobacterium leprae)
-use xylene-peanut oil which enhances acid-fastness -uses Ziel-Neelsen carbol fuchsin solution -carbol fuschin stains the mycobacteria red and the counterstain is methylene blue |
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Auramine O-Rhoadmine B
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-fluorescence technique
-acid-fast organism=reddish-yellow fluorescence -background=black |
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Gram stain
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-demonstrates gram-negative and gram-positive bacteria
-staining solutions are crystal/gentian violet & basic fuchsin -gram (+)=blue; gram(-)=red; background=yellow; nuclei=red |
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Gridley fungus stain
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-demonstrates fungi
-reagents are chromc acid, Schiff reagent, aldehyde fuchsin, & metanil yellow -mycelia=deep purple; conidia=deep rose to purple; background=yellow; elastin & mucin=deep purple |
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Grocott Methenamine Sliver
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-demonstrates fungi & Pneumocystis
-silver nitrate impregnation stain -fungi=delineated in black; background=green |
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Warthin-Starry
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-demonstrates spirochetes
-argyrophil method -spirochetes=black; background=pale yellow to light brown |
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Dieterle
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-demonstrates spirochetes & legionella
-argyrophil method -uses uranyl nitrate (which is radioactive) -spirochetes/bacteria=black; background=pale yellow |
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Steiner & Steiner
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-demonstrates spirochetes, legionella, & H. pylori
-argyrophil method -bacteria=dark brown to black; background=light yellow |
