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11 Cards in this Set
- Front
- Back
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The autoclave works on the principle of what type of heat?
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Moist heat
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What kills the micro-organisms when autoclaving?
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Temperature
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What four things are needed for moist heat sterilization?
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Pressure + moisture + temperature + time = moist heat sterilization
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What 3 things must you check before weighing a substance?
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1. Make sure the balance area does not have excessive air movement
2. Put the balance on a stable bench 3. Set balance back to zero |
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What is the normal temperature of ambient and CO2 incubators?
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35-37 degrees Celsius
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What are 2 advantages of an automated streaker?
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-Streaking is consistent from one streak to another (individuals would vary)
-Automated streaking is time saving |
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What pattern does automated streaking look like?
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Spiral
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What are 4 types of anaerobic organisms?
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1. Faculatative-bacteria will grow equally well with or without O2
2. Strict/Obligate-bacteria that will not grow or even survive in O2 3. Aero-tolerant-bacteria that prefer anaerobic conditions but will grow (to some extent) in O2 |
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What are the 3 main areas of an anaerobic chamber?
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Work area, incubator, anti-chamber
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What are the 4 things needed to obtain anaerobiosis?
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1. A container/environment that is well sealed from room air
2. Palladium pellets as a catalyst (removes O2) 3. A generator 4. Indicator to show if anaerobiosis is achieved` |
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What catalyst is used to obtain anaerobiosis?
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Palladium pellets
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