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11 Cards in this Set
- Front
- Back
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Starch Test
Media Target molecule Product of hydrolysis Exoenzyme Add to plate to read Positive indicator |
Starch agar
Starch Glucose α-Amylase Iodine Zone of clearance |
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Lipase Test
Media Target molecule Product of hydrolysis Exoenzyme Positive indicator |
Tributyrin agar
Triglyceride Glycerol + 3 fatty acids Lipase Zone of clearance |
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Casein Test
Media Target molecule Product of hydrolysis Exoenzyme Positive indicator |
Milk agar
Casein Amino acids Casease Zone of clearance |
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Blood Hemolysis
Media Target molecule Product of hydrolysis Exoenzyme Positive indicator |
TSA 5% sheep blood agar
Red blood cell n/a Hemolysin a hemolysis = partial destruction of the RBC β hemolysis = complete destruction of RBC γ hemolysis = no destruction of RBC α hemolysis = greenish tint to media β hemolysis = zone of clearance γ hemolysis = no change in media |
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Gelatin Hydrolysis
Media Target molecule Product of hydrolysis Exoenzyme Special Note Positive indicator |
Gelatin deep
Gelatin Amino acids Gelatinase The gelatin test tube must be refrigerated before reading Liquid after refrigeration The gelatin test tube must be refrigerated before reading |
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Urea Hydrolysis
Media Target molecule Product of hydrolysis ions Exoenzyme pH indicator Positive indicator |
Urea slant
Urea Ammonium ions Urease Phenol Red Hot pink color of media |
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Bile Esculin Hydrolysis
Media Target molecule Product of hydrolysis Exoenzyme Special Note Positive indicator |
Bile esculin slant
Esculin Esculetin + glucose Glycosidase The media is selective and differential Black color of media |
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Exoenzyme:
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an enzyme that is made inside the cell then secreted outside the cell and functions outside of the cell (β-galactosidase is an exoenzyme)
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Zone of clearance:
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area where the target molecule has been destroyed by an exoenzyme
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Selective media:
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media that contains chemicals that prevents the growth of bacteria with certain characteristics
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Differential media:
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media that can differentiate one organism from a group based on some unique characteristic of that organism
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