Study your flashcards anywhere!

Download the official Cram app for free >

  • Shuffle
    Toggle On
    Toggle Off
  • Alphabetize
    Toggle On
    Toggle Off
  • Front First
    Toggle On
    Toggle Off
  • Both Sides
    Toggle On
    Toggle Off
  • Read
    Toggle On
    Toggle Off
Reading...
Front

How to study your flashcards.

Right/Left arrow keys: Navigate between flashcards.right arrow keyleft arrow key

Up/Down arrow keys: Flip the card between the front and back.down keyup key

H key: Show hint (3rd side).h key

A key: Read text to speech.a key

image

Play button

image

Play button

image

Progress

1/63

Click to flip

63 Cards in this Set

  • Front
  • Back
When doing a Negative stain what is being tested
Shape and arrangement background staining
What special genera is a negative stain used for?
Spirochetes
What chemicals are used in a Negative stain
India Ink
What can be determined by the results of a Negative stain
shape and arrangement, size
What is being tested in capsular stain?
Presence of a capsule
What media is used during a capsular stain
none
What chemicals are used in a capsular stain
India ink, crystal violet
What is being tested in a Gram stain?
positive or negative stain thickness of peptidoglcan
What media is used for a Gram stain
none
What chemicals are used for a Gram stain
Gram stain kit.Crystal violet, Grams Iodine, Alcohol, Safranin Red,
What are the results of Gram staining
Postive= Violet
negative= red/pinkish

No cell wall= red
What is being test for in a spore stain
The presence of an endospore spore + or -
What chemicals are used in a spore stain
Malachite green, safranin red,
What special genera are important for Spore staining
Bacillus and Clostidium
What is the media nutrient Agar used for?
Configuration, elevation, and margins. example
round, raised, wavy.
A nutrient broth culture determins what?
1. where the growth is surface, subsurface, or sediment
2. If the material is Turbid(clowdy) granular (small particles shown) or flocculent(masses are floating around)
A gelatin stab culture determins what?
#1 liquefication: If the organism produces gelatinase(gelatin digestive enzyme)
#2 if no liquefacation occure a growth partern is given. Filiform, beaded, arboresent
A nutrient Agar Slant determins what charactoristics
#1 color in reference to pigments
#2 form of how organisim will grow. example Filiform, Echinulate, Effuse, arborescent.
What does the media blood Agar determin?
If the bacteria is hemolytic or non-hemolytic. hemolytic bacteria distroys red blood cells in media making either clear zones(beta) or Green zones (alpha)
What special genera are associated with Blood Agar
Streptococcus and staphylococcus
To determin Oxygen requirments what medium is used?
Thioglycollate
What results are determined by an oxgen requirement test?
Aerobic(growth on surface) Microaerophilic (surface and subsurface) facultavie spotty growth all over medium, Anaerboic (groth at bottom only)
What charactoristics are determined by starch hydrolysis and what will the results show
If the bacteria is capable of hyrolyzing starch, Iodine will turn agar purple. clear zones indicate + starch hydrolysis
What special genera is used for starch hydrolysis
Baciullus subtilis a positive control for the test should show clear zones.
What chemeicals are used for starch hyrdolysis
Grams Iodine
What media is used for starch hydrolysis
Starch agar
What does the Casein hydrolysis test determin
If the bacteria is capable of hydrolyzing casein, a milk protien
What media is used for the Casien hydrolysis test?
Skim milk agar
What chemicals are used in the casien hydrolysis test
none.
What special genera is used in the Casien hydrolysis test?
Bacillus subtilis a postive control will show clear zones
What is being tested for in a fat hydroylis test
if the bacteria is capable of hydrolyzing fat
What media is used in the fat hydroylis test
spirt blue agar
What specil genera is used for fat hydrolysis
Bacillus subtilis for a postive control will show blue precipitate around the growth
what does the Methyl Red Test determin?
If the bacteria will produce large amounts of acid(lowers PH)
What media is used in the Methly Red Test
methyl red-vp broth
What special genera are used for methyl red test
Escherichia, salmonella, Proteus, Aeromonas.
How the results of the methly red test read?
Postive will show red to orangle color. negative will appear yellowish
What does the Urease test determin
if the bacteria used can brake down urea?
how are the results given for a Urease test
Postive will appear pinkish to purple. Negative will appear light orange.
What is the special genera used in the Urease test?
Proteus vulgaris a postive control will appear pinkish to purple in color
What does the Indole test determin?
If the bacteria will hydrolyze tryptophan, producing indole and pyruvic acid/
what media is used in the indole test
Tryptone broth
what chemicals are used for the Indole test?
Kovacs reagent
how will the results read for the Indole test?
Positive will show a red ring at the surface. Negitive a clear or yellowish ring.
What does the Catalase test determin?
Is the bacteria can produce catalase.
what media is used during the Catalse production test?
Agar slant or plates
What chemicals are used in the catalase production test?
Hydrogen peroxide
How are the results given for the Catalase production test
when hydrogen peroxide is added the culture will bubble indicating Postive catalase production
What does the Durham tube Sugar Fermentation test determin?
Which carbohydrates may be utilized for energy. (which sugars are fermented).
What media is used in the Sugar Fermentation test?
Phenol red broth,
glucose
lactose
mannitol
saccharose
how are the results of the sugar fermentation test shown
The media will turn from red to yellow in the presence of acid
The results should record the presence or absence of both the acid and gas. Gas will show bubble in durham tube.
What does the hydrogen Sulfide production test determin?
If hydrogen suilfide is produced.
What media is used in the hydrogen sulfide test?
Kligler's Iron agar stabs(contains ferrious sulfate, will react with hydrogen sulfide.
how do the results of the hydrogen sulfide test read?
Formation of a dark black color a pereciptitate of iron sulfide
What special genera is used in the hydrogen sulfide test?
Proteus vulgaris for postive control.
What does the Citrate Utilization test determin?
If the bacteria can utilize citrate a a sole source of carbon
What media is used for the citrate test
Simmons citrate agar slant
how do the results read for the citrate test?
It will change from green to a deep blue if growth occurs. No growth indicates a negative test.
What special genera is used in the citrate test?
Bacillus subtilus is a control positive.
What is the refrigerator setting in lab?
1deg cel
what is the autoclave settings?
121 deg C and 15psi for 15mins
what is the incubator settings?
25-37deg cel
What is the oven setting
300deg cel