Microbiology Microscope

Front 1

_____ ______ constructed a significantly better microscope like the kind used in labs today.

Back 1

Joseph Lister

Front 2

in a ____ _____ microscope, the image from the objective lens is magnified again by the ocular lens.

Back 2

compound light

Front 3

the ability of the lenses to distinguish fine details btwn two points at a specified distance apart

Back 3

resolution

Front 4

the light-bending ability of a medium

Back 4

refractive index

Front 5

___ ____ is used to direct light through specimen and keep light from bending away from lenses

Back 5

oil immersion

Front 6

____ ____ is a microscope that allows examination of structures inside cells through the use of a special condenser and lenses.

Back 6

phase-contrast

Front 7

____ ____ ____ is a microscope that accunuates diffraction of the light that passes through a specimen; uses two light beams; 3D

Back 7

differential interference contrast

Front 8

____ is a microscope that absorbs UV light; cells may be stained with flourescent dyes

Back 8

flourescence

Front 9

____ microscope uses flourochromes and a laser light to construct 3D images.

Back 9

confocal

Front 10

___ ____ microscpoy measures sound waves; used to study cells attached to the surface

Back 10

scanning acoustic

Front 11

_____ microscopy uses electrons instead of light through electromagnetic lens; usues beam of electrons from gun

Back 11

electron

Front 12

___ ___ microscopy views ultra thin sections of cells; light passes through cells, then to electromagnetic lens; stained with heavy metal salts

Back 12

transmission electron

Front 13

___ ___ microscopy uses elctron beam that scans the surface of a cell; 3D

Back 13

scanning electron

Front 14

___ ___ microscopy uses a thin metal probe to scan a cell; reveals bumps along surface; much stronger than electron microscope-sees DNA

Back 14

scanning tunneling

Front 15

___ ___ uses a metal and diamond probe inserted into the cell; 3D

Back 15

atomic force

Front 16

a thin film of material/solution containing microbes ont he surface of a slide

Back 16

smear

Front 17

attaching the microorganism to the slide with heat

Back 17

fixing

Front 18

coloring the microorganism with dye that emphasize certain structures

Back 18

staining

Front 19

in basic dyes, the chromophore is a ___. (positively charged & cover cell component)

Back 19

cation

Front 20

in acidic dyes, the chromophore is a ___. (negatively charged & do not penetrate cell component.)

Back 20

anion

Front 21

staining the background, useful for capsules

Back 21

negative staining

Front 22

an aqueous or alcohol of a single basic dye

Back 22

simple stain

Front 23

a ___ may be used to increase affinity, coat the specimen, or enlarge the specimen.

Back 23

mordant

Front 24

used to differeniate bacteria into gram negative or gram positive

Back 24

gram stain

Front 25

gram ___ bacteria retain the primary stain; tend to be killed by penicilin and detergents

Back 25

positive

Front 26

gram ___ will not retain dye but responds to counterstaining; more resistant to antibiotics

Back 26

negative

Front 27

cells that retain a basic stain in the presence of acid-alcohol are called ___.

Back 27

acid-fast

Front 28

acid fast stains only bind to ___ cell wall.

Back 28

waxy/lipid

Front 29

in acid-fast stains, stain used is called red stain ___.

Back 29

carbolfuschin

Front 30

in endospore staining, ___ is required to drive a stain into endospore.

Back 30

heat

Front 31

flagella staining requires a ___ to make the flagella wide enough to see.

Back 31

mordant