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27 Cards in this Set

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Definition: A process that kills or eliminates all types of micro-organisms, including spores
Sterilization
Definition: A process that kills or removes many micro-organisms, excluding spores
Disinfection
T/F: Sterilization kills spores
True
T/F: Sterilization kills prions
False
T/F: Disinfection kills spores
False
Order from Most Resistant to killing to Least Resistant:

Gram + bacteria
Gram - bacteria
Mycobacteria
Prions
Spores
Viruses
Prions
Spores
Mycobacteria
Gram +
Gram -
Viruses
What are two general methods of microbial control?
physical and chemical methods
Dry heat, autoclave, Ionizing ray and Filtration are all part of what method of microbial control?
physical method
Name four physical methods of sterilization
Dry heat, autoclave, Ionizing ray and Filtration
Name two chemical methods of sterilization
Ethylene oxide (Gas sterilization)
and
Glutaraldehyde
What are the specifications of Dry heat Sterilization
160 degrees C for 2 hr
170 degrees C for 1 hr
What are the specifications for autoclave sterilization?
121 degrees C at 15 psi for 15-20 minutes
or
134 degrees C at 30 psi for 3 min.
What is the most commonly used and efficient method of sterilization in dentistry?
autoclave
How does ethylene oxide sterilization work?
Ethylene oxide is an alkylating agent that damages proteins and nucleic acids
When is Ethylene oxide sterilization used?
For heat and water sensitive objects
How long does Ethylene oxide sterilization take to complete?
3-12 hours to sterilize and 8-12 hours to evaporate the gas
The cold sterilizer
Glutaraldehyde
How do you do glutaraldehyde sterilization?
submerge object for 10 hrs in 2% glutaraldehyde
How does glutaraldehyde kill bacteria?
It is an alkalizing agent (very basic)
How is sterilization assurance best determined?
by culturing bacterial spores (usually non-pathogenic Bacillus spores)
What are two sterilization assurance products used
Spore strips and self-contained spores
What are four chemicals used to disinfect?
Formaldehyde, alcohol, oxidizing agents, and quaternary ammonium compounds
most widely used antiseptic
alcohol
Difference between disinfectants and antiseptics
Disinfectants destroy microorganisms in non-living objects. Antiseptics destroy them on living tissue
How is milk pasteurized
by heating to 63 degrees for 30 minutes
The goal of this process is to achieve a logarithmic reduction of organisms in the solution
pasteurization
This process aims to destroy harmful bacteria without changing the composition, flavor, or nutritive value of the liquid
Pasteurization