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What is Microbiology?

The study of small life

What small life is studied in Microbiology?

Microbes and Microorganisms

2 answers

Name two divisions of Prokaryotes.

Bacteria & Archaea

B & A

What is the smallest known microbes?

Viruses

Name four Eukarya divisions

Algae, plants, Fungi, animals, protists, Heminths ( worms), Protozoa

What is a virus?

Microbe.


Acellular Infectious particles.


Most are not visible using light microscopes.

What is an Archaea?

Technically Prokaryotes, Single celled, microbes, many are extremophiles. . . Microbe

Describe Protoza

Single celled 'animals'


Eukaryotes


Microbe


Much larger than bacteria

Describe Fungi

Unicellular and multicellular


Eukaryotes


Examples: yeast and molds


Macroscopic: Mushrooms

Describe Helminths

Worms, multicellular eukaryotic parasite.


Can be micro as an egg, adult can be macro

What is a microbial community?

Biofilm

What are the three domains of life?

Bacteria, archaea, and Eukarya

How many times had microbes changed the world and how?

Twice


1. Great oxygenation


2. Nitrogen cycle

What is a Prokaryote?

Its a cell with a pre-nucleus aka a simple cell

What is the endosymbiotic theory?

States that mitochondrial and Chloroplasts derived from ancient Prokaryotic organisms.


Own DNA


Own proteins


Replicate binary fission

What bacteria can eat through rocks

Lithotrophs

Name four historical infectious diseases.

Plague


Malaria


Cholera


Tuberculosis


Small pox

What is the difference between a pathogen and a parasite?

Pathogens infect organisms and kill the host, parasites feed of their host and need the host to survive.

Who invented the Microscope?


A. Robert Hooke


B. Louis Pasteur


C. Robert Koch


D. Antonie van Leeuwenhoek

A. Robert Hooke


D. Antonie van Leeuwenhoek

How did Louis pasteur disprove spontaneous generation?

Pasteur used a swan-necked flask with boiled chicken broth.

Who is Robert Koch?

Robert Koch discovered microbes caused diseases. He also established Koch's Postulates.

What are Koch's Postulates?

1. Disease causing microbes must only be present in the sick, not the healthy.


2. Microbe must be grown in pure culture.


3. Microbe introduced in new host, host gets sick.


4. Same microbe must be re-isolated from new host.

Which disease microbes did Koch discover?

Anthrax, TB, and Cholera

What did Joseph Lister introduce?

Aseptic techniques

What did Edward Jenner help immunize and by what method?

Edward Jenner took the scabs of cowpox patients and crushed them and gave it to children to protect them against small pox.

Who discover the immunization to rabies?

Louis Pasteur

Who discovered the theory of Viruses?

Dmitri Ivanovsky

Who discover the first antibiotic and how?

Alexander Fleming, he left a plate out which grew bacteria and mold. It was purely an accidently discovery.

Define detection

Ability to see the presence of an object

Define Magnification

Increase in the apparent size of an image

Define resolution

Smallest distance by which two objects can be seperated and still be distinguished.

What is the resolution of the human retina?

150 um

High energy = ?

Higher resolving power

What is empty magnification?

Magnifying with the same resolution

What are the four ways magnification can be altered?

Absorbed, Reflection, Refraction, Scattered

How does one determine the magnification of a microscope?

Magnification of objective lens x Magnification of Ocular lens

What does NA stand for?

Numerical Aperture

What does n stand for in the equation: n sin theta

The n equals the refractive index of the material ex. Air has a value of 1 and oil has a value of 1.5

What is the equation to determine NA?

NA= n sin theta

What is the equation for Resolution (R)?

R=wavelength (^)/(2×NA)

What does TEM stand for

Transmission electron microscopy

What are the two major types of electron microscopy?

Transmission electron microscopy (TEM)


Scanning electron microscopy (SEM)

Extra credit question: What bacteria was mentioned in the first class?

Supersize bacteria - Thiomargarita namibiensis

Its size was key

Who was the first person to discover moving microbes?

Antonie van Leeuwenhoek